Unknown,Transcriptomics,Genomics,Proteomics

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Transcript Profiling of Arcuate Nucleus in Response to Electroacupuncture on Rats

ABSTRACT: The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and non responders (NR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in arcuate nucleus induced by 2Hz/100Hz electroacupuncture in HR and NR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in arcuate nucleus. Rats were exposed to different frequencies (2Hz or 100Hz) electroacupuncture stimulation for 30 min and nociceptive testing and returned to home cages for 1 hour before sacrificed. According to the sensitivity of the analgesic response to 2Hz or 100Hz EA stimulation, the rats divided into four groups: 2Hz-HR group, 2Hz-NR group, 100Hz-HR group and 100Hz-NR group. Subsequently analyzed their dorsal horn transcript profile using cDNA microarrays, the rats were without receiving electroacupuncture as control. The tissue of arcuate nucleus (Arc) of three rats was selected for transcript analysis in each group. The three control rats were mixed and labeled with cy5, each rat of experiment groups was labeled with cy3.

ORGANISM(S): Rattus norvegicus

SUBMITTER: ke wang

PROVIDER: E-GEOD-30365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The electroacupuncture-induced analgesic effect has been used widely to alleviate diverse pains. However, significant individual variations in analgesic effect of EA for both experiments and clinics were reported. According to the sensitivity of the analgesic response to EA stimulation, the subjects could be categorized into high responders (HR) and low responders (LR). However, the molecular mechanism of individual variability in the analgesic response to acupuncture stimulation is still uncertain. This study aims to investigate the potential gene expression in spinal dorsal horn induced by 2Hz/100Hz electroacupuncture in HR and LR rats. Rats were given 2Hz or 100Hz electroacupuncture for 30 min and using cDNA microarrays to compare different gene expression in dorsal horn. Transcriptome profiling analysis found that some co-regulated genes related with electroacupuncture or 2Hz or 100Hz freqencies. These co-regulated genes were plasticity-related by GO analysis. We also found some special regulated genes in HR vs. LR in 2Hz/100Hz electroacupuncture stimulation. These results suggested that neurotransmitter system and cytokine different between HR with LR in 2Hz electroacupuncture. But in 100Hz electroacupunture, there were many different regulated genes related with ribosome between HR with LR, which needs more studies to research the function and may play an important role in HR vs. LR by 100Hz electroacupuncture. Keywords: Transcriptome analysis Rats were exposed to different frequencies (2Hz or 100Hz) electroacupuncture stimlation for 30 min and nociceptive testing and returned to home cages for 1 hours before sacrificed. According to the sensitivity of the analgesic response to 2Hz or 100Hz EA stimulation, the rats divided into four groups: 2Hz-HR group, 2Hz-LR group, 100Hz-HR group and 100Hz-LR group. Subsequently analyzed their dorsal horn transcript profile using cDNA microarrays, the rats were without receiving electroacupuncture as control. The tissue of dorsal horn (DH) of the fifth and sixth lumbar (L5 and L6) of four or five rats were selected for transcript analysis in each group. The five control rats were mixed and labeled with cy5, each rat of experiment groups was labeled with cy3.

Project description:It generally believes that moderate stress could have protective and adaptive effects, but less is known about the moderate or experimental stress on CNS in stimulated animals. This study aims to investigate the potential gene expression in nociception-related central regions induced by moderate experimental stress in rats. Rats were given moderate restrain for 50 min and using cDNA microarrays to compare different gene expression in central regions. Transcriptome profiling analysis showed that stress-related genes at acute stage were up-regulated in Arc; rhythmic process-related genes were up-regulated in PAG, while glutamine metabolism-, arginine metabolis-related genes were up-regulated in DH. At late stage, immune related genes were broadly up-regulated in the CNS regions. These results suggest that specific GO categories? genes could be regulated in the specific CNS regions correlated with relative function in response to external stimuli at the beginning period. It also suggest that moderate stress could enhance neuroendocrine and immune function in broad CNS regions at late stage, and experimental stress should be careful considered, especially in neuroendocrine and immune studies. Furthermore, Sgk1 was verified by qRT-PCR and western blot. The significant up regulation implied Sgk1 in the CNS may play an important role in the response to stress. Keywords: Transcriptome analysis Rats were exposed to moderate restrain for 50 min and nociceptive testing and returned to home cages for 1 hours or 24 hours before sacrificed, termed as restrain_nociceptive_test_group 1 (RT1) and restrain_nociceptive_test_group 24 (RT24) respectively. Subsequently analyzed their nociception-related central regions transcript profile using cDNA microarrays, the rats were without receiving restraint stress and nociceptive testing as control. The tissue of arcuate nucleus (Arc), periaqueductal gray (PAG) and dorsal horn (DH) of the fifth and sixth lumbar (L5 and L6) of five rats were selected for transcript analysis in each group. The five control rats were mixed and labeled with cy5 according to different CNS regions, each RT1 or RT24 rats were labeled with cy3.

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Transcript Profiling of Arcuate Nucleus in Response to Electroacupuncture on Rats

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