Unknown,Transcriptomics,Genomics,Proteomics

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Murine IgD+ B Cells transcriptome analysis when stimulated with LPS and LPS+anti-CD40 [72 hours]


ABSTRACT: The effect of LPS and LPS+antiCD40 on the Transcriptome of IgD+ B Cells was analysed here at 72 hours after the stimulation. B-lymphocytes constitute an important aspect of mammalian immune systems by virtue of their ability to produce highly specific antibodies in response to foreign antigens and pathogens. When B-lymphocytes encounter the particular antigen that they are responsive to, they differentiate into two types of cells: short-lived antibody-secreting plasma cells and long-lived memory cells. While the plasma B cells are responsible for the rapid resolution of a current infection or immune challenge, the memory B cells are responsible for mounting a rapid response to subsequent exposures. Previous work had demonstrated that treatment of mice with a single dose of anti-CD40 at the time of immunization lead to improved secondary responses, suggesting an enhancement of the memory B cell pool. We are currently studying the molecular mechanisms underlying the generation of memory B cells, using CD40 signalling as a tool. We have carried out a Microarray analyses of genome wide gene expression patterns in naïve B-lymphocytes following CD40 signalling, over multiple time points. Analysis used LPS treated IgD+ B cells as control for comparison to LPS+anti-CD40 treated cells (test).Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.

ORGANISM(S): Mus musculus

SUBMITTER: Tushar Vaidya 

PROVIDER: E-GEOD-30412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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