Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of mRNA expression among rat hepatoma cell lines with different metastatic ability

ABSTRACT: To reduce cancer mortality, understanding of mechanisms of cancer metastasis is crucial. We have established 6 rat hepatocellular carcinoma (HCC) cell lines, which exhibit differing metastatic potential to the lung after inoculation into the tail veins of nude mice. Micorarray analysis of 4 kinds of HCC cells and rat normal liver tissue was performed to find a potent molecular target for prevention of cancer metastasis. By the microarray analysis, mRNA expression was compared among two low-metastatic rat HCC cell lines (C5F and C6) and two high-metastatic rat HCC cell lines (N1 and L2) as well as non-treated rat liver tissue (as a reference sample).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Kumiko Ogawa

PROVIDER: E-GEOD-30469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Silencing of connexin 43 suppresses invasion, migration and lung metastasis of rat hepatocellular carcinoma cells.

Ogawa Kumiko K Pitchakarn Pornsiri P Suzuki Shugo S Chewonarin Teera T Tang MingXi M Takahashi Seishiro S Naiki-Ito Aya A Sato Shinya S Takahashi Satoru S Asamoto Makoto M Shirai Tomoyuki T

Cancer science 20120328 5

To reduce cancer mortality, understanding of mechanisms of cancer metastasis is crucial. We have established six rat hepatocellular carcinoma (HCC) cell lines, which exhibit differing metastatic potential to the lung after inoculation into the tail veins of nude mice. In the present experiment, we investigated the process of cell attachment to metastatic sites and possible regulating factors. One hour after inoculation, two of two HCC cell lines with high metastatic potential and one of two HCC ...[more]

PMID: 22320152

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Project description:Impacts of plasmid carriage on its host cell were comprehensively analyzed using conjugative plasmid pCAR1 in the three different kinds of hosts, Pseudomonas putida KT2440, P. aeruginosa PAO1, and P. fluorescens Pf0-1. Various analyses of the host phenotype showed that pCAR1 carriage reduced host fitness, swimming motility, and resistances to osmotic- or pH-stress, and brought about the alterations of primary metabolic capacities in the TCA cycle or those several steps away from the TCA cycle in the host cells. Growth phase-dependent transcriptome analyses were performed with the classification of the annotated genes based on their identities among the three hosts and their putative functions. pCAR1 carriage affected host transcriptome more greatly at the transition and stationary phases in each host. The transcriptome responses were more similar between KT2440 and PAO1 than between other host combinations, and many genes, such as for ribosomal proteins, F-type ATPase, and RNAP core, in both strains were commonly not suppressed in their stationary phases. These responses may have resulted in the reduction of host fitness, motility, and stress resistances. Host-specific responses to plasmid carriage were transcriptional changes of genes on putative prophage or foreign DNA regions. The extent of the impacts in host phenotypes and transcriptomes was similarly the largest in KT2440 and the lowest in Pf0-1. The host alterations controlled by pCAR1 carriage are important for understanding the fate of the plasmid and its host, plasmid maintenance, expression of plasmid genes, the host cell physiology, and host survivability in the environment. The growth-dependent change of chromosomal RNA maps from exponential to early stationary phases of pCAR1-harboring KT2440 and non-pCAR1 harboring KT2440 were compared. The growth-dependent change of chromosomal RNA maps from exponential to early stationary phases of pCAR1-harboring PAO1 and non-pCAR1 harboring PAO1 were compared. The growth-dependent change of chromosomal RNA maps from exponential to early stationary phases of pCAR1-harboring Pf0-1 and non-pCAR1 harboring Pf0-1 were compared. The growth-dependent change of RNA maps from exponential to early stationary phases of plasmid pCAR1possessed by 3 kinds of hosts were compared.

Dataset Information

Comparison of mRNA expression among rat hepatoma cell lines with different metastatic ability

Publications

Silencing of connexin 43 suppresses invasion, migration and lung metastasis of rat hepatocellular carcinoma cells.

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