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Epigenetic analysis of gastric cancer

ABSTRACT: Genome-wide DNA methylation profiling of gastric tumors and matched gastric non-malignant samples. The Illumina HumanMethylation27 BeadChip was used to obtain DNA methylation profiles across 27,578 CpGs in 203 gastric tumors and 94 matched non-malignant gastric samples. Bisulphite-converted DNA from the 203 gastric tumors and 94 matched gastric non-malignant samples was hybridized to the Illumina HumanMethylation27 BeadChip.

ORGANISM(S): Homo sapiens

SUBMITTER: Patrick Tan

PROVIDER: E-GEOD-30601 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Methylation subtypes and large-scale epigenetic alterations in gastric cancer.

Zouridis Hermioni H Deng Niantao N Ivanova Tatiana T Zhu Yansong Y Wong Bernice B Huang Dan D Wu Yong Hui YH Wu Yingting Y Tan Iain Beehuat IB Liem Natalia N Gopalakrishnan Veena V Luo Qin Q Wu Jeanie J Lee Minghui M Yong Wei Peng WP Goh Liang Kee LK Teh Bin Tean BT Rozen Steve S Tan Patrick P

Science translational medicine 20121001 156

Epigenetic alterations are fundamental hallmarks of cancer genomes. We surveyed the landscape of DNA methylation alterations in gastric cancer by analyzing genome-wide CG dinucleotide (CpG) methylation profiles of 240 gastric cancers (203 tumors and 37 cell lines) and 94 matched normal gastric tissues. Cancer-specific epigenetic alterations were observed in 44% of CpGs, comprising both tumor hyper- and hypomethylation. Twenty-five percent of the methylation alterations were significantly associa ...[more]

PMID: 23076357

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Epigenetic analysis of gastric cancer

Project description:Genome-wide DNA methylation profiling of gastric tumors and matched gastric non-malignant samples. The Illumina HumanMethylation27 BeadChip was used to obtain DNA methylation profiles across 27,578 CpGs in 203 gastric tumors and 94 matched non-malignant gastric samples.

2012-10-24 | GSE30601 | GEO

Genetic Landscape of Copy Number Alterations in Gastric Cancer

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Genetic Landscape of Copy Number Alterations in Gastric Cancer

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A signature predicting poor prognosis in gastric and ovarian cancer represents a coordinated macrophage and stromal-response.

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2014-04-01 | E-GEOD-51105 | biostudies-arrayexpress

The miRNA expression signature of human MALT lymphoma

Project description:To identify key tumour supressor miRNAs involed in MALT lymphoma pathogenesis Gastric mucosa-associated lymphoid tissue lymphoma develops in the chronically inflamed mucosa of Helicobacter pylori-infected patients. MicroRNA expression profiling of human MALT lymphoma revealed a 10-fold down-regulation of miR-203, which resulted from promoter hypermethylation and coincided with the dysregulation of the miR-203 target ABL1. Demethylating treatment of lymphoma B-cells led to an increase in miR-203 expression and concomitant ABL1 down-regulation. The lentiviral delivery of miR-203, as well as treatment with various ABL inhibitors, prevented primary MALT lymphoma cell proliferation in vitro. Finally, the treatment of tumor-bearing mice with imatinib induced MALT lymphoma regression in vivo. Our results show that MALT lymphomagenesis is epigenetically induced by miR-203 promoter methylation and identify ABL1 as a novel target for the treatment of this malignancy. 5 human fresh frozen MALT lymphoma samples were analysed and 4 human tonsil tissue samples were used as the non-tumour control

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