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Transcription profiling of human Stratagene Universal RNA on Affymetrix U133 Plus 2.0 and U133 A Chips

ABSTRACT: Stratagene universal human RNA hybridized to both U133 Plus 2.0 and U133 A arrays. Experiment Overall Design: Stratagene universal human RNA was processed using 5 aliquots of 2 ug each. The samples were hybridized to both 133 Plus 2.0 and 133 A arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Sean Yoder

PROVIDER: E-GEOD-3061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identical probes on different high-density oligonucleotide microarrays can produce different measurements of gene expression.

Zhang LanMin L Yoder Sean J SJ Enkemann Steven A SA

BMC genomics 20060615

<h4>Background</h4>There are many potential sources of variability in a microarray experiment. Variation can arise from many aspects of the collection and processing of samples for gene expression analysis. Oligonucleotide-based arrays are thought to minimize one source of variability as identical oligonucleotides are expected to recognize the same transcripts during hybridization.<h4>Results</h4>We demonstrate that although the probes on the U133A GeneChip arrays are identical in sequence to pr ...[more]

PMID: 16776839

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