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Gene expression change after LSD1 siRNA treatment in ER-negative breast cancer cells MDA-MB-231

ABSTRACT: Knock-down of LSD1 using siRNA approach induced regulation of several proliferation-associated genes in ER-negative breast cancer cells MDA-MB-231. To identify changes on gene expression caused by treatment with siRNA directed against LSD1 (si) or control siRNA (control) in MDA-MB-231 cells, total RNA was purified from the cells after treatment for 6 days (2 rounds of transfection). Three biological replicates were used.

ORGANISM(S): Homo sapiens

SUBMITTER: Soyoung Lim

PROVIDER: E-GEOD-30775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components.

Janzer Andreas A Lim Soyoung S Fronhoffs Florian F Niazy Naima N Buettner Reinhard R Kirfel Jutta J

Biochemical and biophysical research communications 20120417 4

Histone modifying enzymes confer epigenetic marks, directing the changes in gene expression required for diverse cellular processes. Lysine-specific demethylase 1 (LSD1) functions as a transcriptional coregulator by demethylating histone H3 on lysine 4 and lysine 9. Analyzing transcriptomes on microarrays, we identified genes which represent inflammatory-related targets of LSD1. We demonstrate a repressive role of LSD1 in proinflammatory cytokine expression such as IL1α, IL1β, IL6 and IL8 and cl ...[more]

PMID: 22542627

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