Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Role of Interleukin-6 in Influenza Pandemic A (H1N1pdm) Infection in Mice

ABSTRACT: Profile of host response in C57BL6/J mice infected with A/Mexico/4108/2009 (H1N1), spanning days 1 through 6 post-infection. Focused analysis on the involvement of IL-6 in the host response. Total RNA obtained from lung tissue collected from infected C57BL6/J mice at t = 1, 2, 3, 4, 5, 6 days post-infection (n=3/time point). Uninfected control samples were also obtained (n=3).

ORGANISM(S): Mus musculus

SUBMITTER: Longsi Ran

PROVIDER: E-GEOD-31022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Interleukin-6 is a potential biomarker for severe pandemic H1N1 influenza A infection.

Paquette Stéphane G SG Banner David D Zhao Zhen Z Fang Yuan Y Huang Stephen S H SS Leόn Alberto J AJ Ng Derek C K DC Almansa Raquel R Martin-Loeches Ignacio I Ramirez Paula P Socias Lorenzo L Loza Ana A Blanco Jesus J Sansonetti Paola P Rello Jordi J Andaluz David D Shum Bianche B Rubino Salvatore S de Lejarazu Raul Ortiz RO Tran Dat D Delogu Giovanni G Fadda Giovanni G Krajden Sigmund S Rubin Barry B BB Bermejo-Martin Jesús F JF Kelvin Alyson A AA Kelvin David J DJ

PloS one 20120605 6

Pandemic H1N1 influenza A (H1N1pdm) is currently a dominant circulating influenza strain worldwide. Severe cases of H1N1pdm infection are characterized by prolonged activation of the immune response, yet the specific role of inflammatory mediators in disease is poorly understood. The inflammatory cytokine IL-6 has been implicated in both seasonal and severe pandemic H1N1 influenza A (H1N1pdm) infection. Here, we investigated the role of IL-6 in severe H1N1pdm infection. We found IL-6 to be an im ...[more]

PMID: 22679491

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Project description:Background: Pandemic H1N1 influenza A is a newly emerging strain of human influenza that is easily transmitted between people and has spread globally to over 116 countries. Human infection leads to symptoms ranging from mild to severe with lower respiratory complications observed in a small but significant number of infected individuals. Little is currently known about host immunity and Pandemic H1N1 influenza infections. Methods: We examined the pathogenic potential of the pandemic influenza A vaccine strain, A/California/07/2009 (H1N1), in ferrets, and characterized the host immune responses using microarray analysis. Gene expression profiles in lung tissue were compared with those from ferrets infected with A/Brisbane/59/2007. Results: Chemokines CCL2, CCL8, CXCL7 and CXCL10 along with the majority of ISGs were expressed early, correlated to lung pathology, and abruptly decreased expression in 5 days. Interestingly, the drop in innate immune gene expression was replaced by a significant increase in the expression of the adaptive immune genes for granzymes and immunoglobulins. Serum anti-pandemic influenza H1N1 antibodies were also observed on day 7, commensurate with the elimination of viral load. Conclusions: We propose that the innate phase of host immunity causes lung pathology and a delay or failure to effectively switch to the adaptive phase contributes to morbidity and mortality during severe human pandemic H1N1 influenza A infections. Keywords: influenza, immune response, cytokines, chemokines, lung infection, time course In the experiment with influenza A/California/07/2009 (H1N1),15 ferrets were randomly allocated to 5 groups: Day 0 (before infection), and Day 3, 5, 7 and 14 (post infection) with 3 biological replicates for each group. Likewise, a second experiment with A/Brisbane/59/2007 (H1N1) was carried out using the same experimental groups, except for a group in Day 2, instead Day 3. Ferrets were euthanized and lung tissue was excised for RNA purification on the scheduled date. The subsequent gene expression analysis was performed with Affymetrix GeneChip Canine Genome 2.0 Array. Day 0 groups were used as control.

Dataset Information

Role of Interleukin-6 in Influenza Pandemic A (H1N1pdm) Infection in Mice

Publications

Interleukin-6 is a potential biomarker for severe pandemic H1N1 influenza A infection.

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