Unknown,Transcriptomics,Genomics,Proteomics

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Small RNA sequencing in normal and psoriatic skin


ABSTRACT: We report the application of Illumina small RNA sequencing to normal human skin, as well as uninvolved and involved psoriatic skin. By obtaining over 600 million qualified reads from 20 healthy controls and 47 psoriasis biopsies (uninvolved/involved), we have generated a complete small RNA profile in normal and diseased human skin, with particular emphasis on miRNAs. We report the discovery of 284 putative novel miRNAs as well as 98 differentially expressed miRNAs in psoriatic skin. miRNA discovery and expression profiling in 67 normal and psoriatic human skin biopsies

ORGANISM(S): Homo sapiens

SUBMITTER: Xiang Zhou 

PROVIDER: E-GEOD-31037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Deep sequencing of small RNAs from human skin reveals major alterations in the psoriasis miRNAome.

Joyce Cailin E CE   Zhou Xiang X   Xia Jing J   Ryan Caitriona C   Thrash Breck B   Menter Alan A   Zhang Weixiong W   Bowcock Anne M AM  

Human molecular genetics 20110801 20


Psoriasis is a chronic and complex inflammatory skin disease with lesions displaying dramatically altered mRNA expression profiles. However, much less is known about the expression of small RNAs. Here, we describe a comprehensive analysis of the normal and psoriatic skin miRNAome with next-generation sequencing in a large patient cohort. We generated 6.7 × 10(8) small RNA reads representing 717 known and 284 putative novel microRNAs (miRNAs). We also observed widespread expression of isomiRs and  ...[more]

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