Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from GW8510 treatment of pancreatic cells


ABSTRACT: Expression of insulin in terminally differentiated non-beta pancreatic cell types could be important for treating type-1 diabetes. We observed that the kinase inhibitor GW8510 up-regulated insulin expression in mouse pancreatic alpha cells. Gene-expression profiling and gene-set enrichment analysis of GW8510-treated alpha cells suggested coordinate up-regulation of the p53 pathway, which was further implicated in induction of insulin transcript. In order to determine a potential mechanism of insulin induction by GW8510, we treated mouse alpha cells (aTC1) and beta cells (bTC3) with 3.3 µM GW8510 or 0.1% DMSO for five days, and performed gene-expression profiling followed by gene-set enrichment analysis (GSEA). Three biological replicates were included per condition. GW8510 treatment in each cell line was compared to DMSO-treated control.

ORGANISM(S): Mus musculus

SUBMITTER: Dina Fomina-Yadlin 

PROVIDER: E-GEOD-31102 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-03-09 | E-GEOD-36379 | biostudies-arrayexpress
2014-08-05 | E-GEOD-54709 | biostudies-arrayexpress
2012-12-14 | E-GEOD-42897 | biostudies-arrayexpress
2014-07-24 | E-GEOD-57227 | biostudies-arrayexpress
2015-01-29 | E-GEOD-65398 | biostudies-arrayexpress
2009-12-31 | E-GEOD-15866 | biostudies-arrayexpress
2015-06-18 | E-GEOD-68317 | biostudies-arrayexpress
2013-08-28 | E-GEOD-50386 | biostudies-arrayexpress
2014-03-25 | E-GEOD-56130 | biostudies-arrayexpress
2011-08-02 | GSE31102 | GEO