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Expression data from inducible ES stable cell line

ABSTRACT: In order to identify the effects of the induction of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line. Transcriptome analysis of the inducible transgenic mouse ES cell line. The E13 inducible cell lines derived from parental EB3 cell line. The cell line EB3 was obtained from the laboratory of Dr Hitoshi Niwa as previously described in (Masui S et al., 2005). Tthe specific mouse gene is E130012A19Rik. For the analysis on the inducible not-tagged cell line, total RNA was extracted from three biological replicates grown in medium deprived of Tetracycline for 48 hours; RNA extracted from un-induced clones was used as control.

ORGANISM(S): Mus musculus

SUBMITTER: Rossella De Cegli 

PROVIDER: E-GEOD-31166 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.

De Cegli Rossella R   Iacobacci Simona S   Flore Gemma G   Gambardella Gennaro G   Mao Lei L   Cutillo Luisa L   Lauria Mario M   Klose Joachim J   Illingworth Elizabeth E   Banfi Sandro S   di Bernardo Diego D  

Nucleic acids research 20121123 2

Gene expression profiles can be used to infer previously unknown transcriptional regulatory interaction among thousands of genes, via systems biology 'reverse engineering' approaches. We 'reverse engineered' an embryonic stem (ES)-specific transcriptional network from 171 gene expression profiles, measured in ES cells, to identify master regulators of gene expression ('hubs'). We discovered that E130012A19Rik (E13), highly expressed in mouse ES cells as compared with differentiated cells, was a  ...[more]

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