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SRNA sequencing from wild-type Arabidopsis (diverse tissue types)

ABSTRACT: This set consists of small RNAs sequenced from wild-type Arabidopsis samples from multiple tissue types (floral, leaf, seedling, silique). Samples were prepared in two different labs and presumably under different (unknown) growth conditions, hence type A (UEA) and type B (University of Cambridge) samples. 4 biological replicates (7 technical replicates) of Floral A; 3 biological replicates of Floral B; 1 biological replicate of Leaf B; 2 biological replicates of Leaf A; 3 biological replicates of Seedling; 1 biological replicate of Silique. Note that GSM415783, GSM415784 and GSM415785 form part of the 'Floral A' category; GSM415783, GSM415784 are technical replicates.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Krys Kelly

PROVIDER: E-GEOD-31211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identifying small interfering RNA loci from high-throughput sequencing data.

Hardcastle Thomas J TJ Kelly Krystyna A KA Baulcombe David C DC

Bioinformatics (Oxford, England) 20111209 4

<h4>Motivation</h4>Small interfering RNAs (siRNAs) are produced from much longer sequences of double-stranded RNA precursors through cleavage by Dicer or a Dicer-like protein. These small RNAs play a key role in genetic and epigenetic regulation; however, a full understanding of the mechanisms by which they operate depends on the characterization of the precursors from which they are derived.<h4>Results</h4>High-throughput sequencing of small RNA populations allows the locations of the double-st ...[more]

PMID: 22171331

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