Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from the partially synthetic yeasts SynIXR-1D, -6B, -22D, and controls.

ABSTRACT: We have replaced the right arm of chromosome IX in Saccharomyces cerevisiae with a synthetic version to generate synIXR haploids. The synthetic chromosome features multiple sequeunce modifications. We analyzed gene expression by microarray analysis in three synIXR haploids (1D, 6B, and 22D) to detect any changes in synIXR transcripts or global compensatory changes. Biological triplicates of three synIXR haploids (1D, 6B, and 22D) and corresponding controls (either BY4741 or BY4742) were grown to mid-log phase and collected for mRNA isolation.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jessica Dymond

PROVIDER: E-GEOD-31326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design.

Dymond Jessica S JS Richardson Sarah M SM Coombes Candice E CE Babatz Timothy T Muller Héloïse H Annaluru Narayana N Blake William J WJ Schwerzmann Joy W JW Dai Junbiao J Lindstrom Derek L DL Boeke Annabel C AC Gottschling Daniel E DE Chandrasegaran Srinivasan S Bader Joel S JS Boeke Jef D JD

Nature 20110914 7365

Recent advances in DNA synthesis technology have enabled the construction of novel genetic pathways and genomic elements, furthering our understanding of system-level phenomena. The ability to synthesize large segments of DNA allows the engineering of pathways and genomes according to arbitrary sets of design principles. Here we describe a synthetic yeast genome project, Sc2.0, and the first partially synthetic eukaryotic chromosomes, Saccharomyces cerevisiae chromosome synIXR, and semi-synVIL. ...[more]

PMID: 21918511

Dataset Information

Expression data from the partially synthetic yeasts SynIXR-1D, -6B, -22D, and controls.

Publications

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design.

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