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MiRNA expression profiling of NKTCL (Natural Killer/T-Cell Lymphoma) samples

ABSTRACT: We performed a comprehensive genome-wide miRNA expression profiling (MEP) of extranodal nasal-type Natural Killer/T-cell lymphoma (NKTL) using formalin fixed paraffin-embedded tissue (FFPE) (n=30) and NK cell lines (n=6) in comparison with normal NK cells, with the objective of understanding the pathogenetic role of miRNA deregulation in NKTL. Total RNA, including miRNA, were extracted using Ambion Recoverall Kit and profiled using Agilent human miRNA V2.

ORGANISM(S): Homo sapiens

SUBMITTER: Chong-Lei Bi

PROVIDER: E-GEOD-31377 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma.

Ng Siok-Bian SB Yan Junli J Huang Gaofeng G Selvarajan Viknesvaran V Tay Jim Liang-Seah JL Lin Baohong B Bi Chonglei C Tan Joy J Kwong Yok-Lam YL Shimizu Norio N Aozasa Katsuyuki K Chng Wee-Joo WJ

Blood 20110914 18

We performed a comprehensive genome-wide miRNA expression profiling of extranodal nasal-type natural killer/T-cell lymphoma (NKTL) using formalin-fixed paraffin-embedded tissue (n = 30) and NK cell lines (n = 6) compared with normal NK cells, with the objective of understanding the pathogenetic role of miRNA deregulation in NKTL. Compared with normal NK cells, differentially expressed miRNAs in NKTL are predominantly down-regulated. Re-expression of down-regulated miRNAs, such as miR-101, miR-26 ...[more]

PMID: 21921041

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