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Genome-wide maps of methylation state in cisplatin sensitive and resistant ovarian cancer cell line

ABSTRACT: We report the application of methyl-binding protein sequencing (MBD-seq) to global identify aberrant methylation between cisplatin sensitive and resistant ovarian cancer cell lines Detection of aberrant methylation states between cisplatin sensitive and resistant cell lines

ORGANISM(S): Homo sapiens

SUBMITTER: Biao Yang Lin

PROVIDER: E-GEOD-31418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global analysis of DNA methylation by Methyl-Capture sequencing reveals epigenetic control of cisplatin resistance in ovarian cancer cell.

Yu Wei W Jin Chengmeng C Lou Xiaoyan X Han Xu X Li Lisha L He Yinghua Y Zhang Hongyu H Ma Kelong K Zhu Jingde J Cheng Lihua L Lin Biaoyang B

PloS one 20111222 12

Cisplatin resistance is one of the major reasons leading to the high death rate of ovarian cancer. Methyl-Capture sequencing (MethylCap-seq), which combines precipitation of methylated DNA by recombinant methyl-CpG binding domain of MBD2 protein with NGS, global and unbiased analysis of global DNA methylation patterns. We applied MethylCap-seq to analyze genome-wide DNA methylation profile of cisplatin sensitive ovarian cancer cell line A2780 and its isogenic derivative resistant line A2780CP. W ...[more]

PMID: 22216282

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