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Expression data from HeLa cells transfected with SLC44A5

ABSTRACT: We identified SLC44A5 as a gene associated with birth weight in cattle based on genome wide association studies. To identify the mechanisms involved in growth and choline transporter, we performed genome-wide mRNA expression analysis in HeLa cells transfected with SLC44A5. HeLa cells were transfected with negative control, siRNA against SLC44A5, bovine SLC44A5 expression plasmid, and an empty vector control for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Mayumi Sugimoto

PROVIDER: E-GEOD-31434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from HeLa cells transfected with SLC44A5

Project description:We identified SLC44A5 as a gene associated with birth weight in cattle based on genome wide association studies. To identify the mechanisms involved in growth and choline transporter, we performed genome-wide mRNA expression analysis in HeLa cells transfected with SLC44A5.

2012-09-17 | GSE31434 | GEO

Analysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.

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SF3b155 knock-down by two different siRNA oligos in HeLa cells

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Role of HIP1 in HIPPI mediated transcription regulation in HeLa cells

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Exon arrays from HeLa cells transfected with RBM4-specific siRNA

Project description:RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10M-bM-^@M-^S15% of WilmsM-bM-^@M-^Y tumours and more recently in leukemya, is considered to be a tumour suppressor. RBM4, a RBP whose splicing effect is inhibited by the +KTS isoform of the tumour-suppressor/activator WT1, exhibits altered expression in different tumours, and may be essential for proliferation. Moreover, RBM4 binds to a number of RNAs of genes involved in acute myeloid leukaemia and cell cycle control. These results suggest that RBM4 may be involved in alternative splicing, tumorigenesis and particularly leukaemogenesis. To determine the endogenous transcripts that are targeted by RBM4 at the genome-wide level, we decided to perform exon microarray studies. RBM4-specific siRNA has been used for knockdown of RBM4 in HeLa cells. RNA was extracted using a Qiagen RNA extraction kit from untransfected, mock and siRNA-transfected HeLa cells and quality of RNA for microarray analysis was checked using a Bioanalyzer 2100 (Agilent Technologies). Experiments were run in triplicates.

2012-07-14 | E-GEOD-32933 | biostudies-arrayexpress

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2009-02-21 | E-GEOD-13639 | biostudies-arrayexpress

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2014-07-18 | E-GEOD-53279 | biostudies-arrayexpress

Exon arrays from HeLa cells transfected with GFP-ZRANB2 vector

Project description:Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3M-bM-^@M-^Y ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7). It has been shown to alter splicing patterns of Tra2M-NM-21 minigene primary transcripts in a dose-dependent manner. It is still unclear what role ZRANB2 plays in the regulation of alternative splicing. To determine the endogenous transcripts that are targeted by ZRANB2 at the genome wide level, we decided to perform exon microarray studies and analyzed the suitability of this method to examine splicing differences in human cells overexpressing a splicing factor. GFP-ZRANB2 construct containing isoform 2 (short form, SF) of ZRANB2 was used. This construct includes exon 1 through to alternative exon 10. Isoform 2 was chosen since it has been shown previously to affect alternative splicing of a Tra2M-NM-21 minigene. HeLa cells were transfected with GFP-ZRANB2, control vector GFP or were left untransfected. RNA was extracted using a Qiagen RNA extraction kit. Experiments were run in 5 replicates.

2012-12-15 | E-GEOD-32932 | biostudies-arrayexpress

Expression data from HeLa cells transiently transfected with control siRNA or SON siRNA

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2011-04-01 | E-GEOD-26888 | biostudies-arrayexpress

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