Unknown,Transcriptomics,Genomics,Proteomics

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Effect of endoplasmic reticulum stress on gene expression in lymphoblastoid cell lines.


ABSTRACT: The ER stress inducing agent Thapsigargin (TG) and/or the cytoprotective agent Salubrinal were applied to lymphoblastoid cell lines. TG induced lytic replication as well as a distinct pattern of gene expression changes. This study was designed to identify host genes mediating lytic replication secondary to ER stress. DMSO or TG applied for 6 hrs then washed out, immediately followed by either (i) DMSO, or (ii) SAL resulting in 4 conditions (DMSO, TG, SAL, or TG+SAL). Total RNA harvested at 3, 24 or 72hrs for Affymetrix analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Adam Rosendorff 

PROVIDER: E-GEOD-31447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Endoplasmic reticulum stress causes EBV lytic replication.

Taylor Gwen Marie GM   Raghuwanshi Sandeep K SK   Rowe David T DT   Wadowsky Robert M RM   Rosendorff Adam A  

Blood 20110817 20


Endoplasmic reticulum (ER) stress triggers a homeostatic cellular response in mammalian cells to ensure efficient folding, sorting, and processing of client proteins. In lytic-permissive lymphoblastoid cell lines (LCLs), pulse exposure to the chemical ER-stress inducer thapsigargin (TG) followed by recovery resulted in the activation of the EBV immediate-early (BRLF1, BZLF1), early (BMRF1), and late (gp350) genes, gp350 surface expression, and virus release. The protein phosphatase 1 a (PP1a)-sp  ...[more]

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