Tea tree oil induced transcriptional alterations in Staphylococcus aureus
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ABSTRACT: S. aureus and MRSA are susceptible to TTO and are therefore targeted in nascent TTO clinical trails. We now report the alterations in the transcriptome of a S. aureus exposed to a growth inhibitory concentration of TTO. These efforts have uncovered additional mechanisms by which this membrane active antimicrobial substance inhibits bacterial growth. Four hybridizations, including a biological replicate and a dye-swap experiment for each replicate was carried out. LOWESS normalization was performed on the raw data to correct for dye-bias. Statistical analysis was performed using a significance analysis of microarrays (SAM, MultiExperiment Viewer, ver. 4.0) unpaired contrast. A d-statistic was calculated for each gene based on repeated permutations, and a false discovery rate FDR of 0.01 was used to assign a critical cutoff for significance.
ORGANISM(S): Staphylococcus aureus
SUBMITTER: John Gustafson
PROVIDER: E-GEOD-31554 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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