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Ecological success of a group of Saccharomyces cerevisiae / Saccharomyces kudriavzevii hybrids in the Northern European wine making environment.

ABSTRACT: The aim of this project was to evaluate the ploidy of a S. cerevisiae *S. kudriavzevii hybrid in comparison to the lab strain S288C. Other wine yeast have been icluded in the project for the global analysis. Comparison of Eg8 (2 arays) , to S288C (3 arrays), other strains V5, U13, Eg25 (2 arrays) are used to enable signal normalization acccross arrays with RMA package.

ORGANISM(S): Saccharomyces cerevisiae x Saccharomyces kudriavzevii

SUBMITTER: Jean-Luc Legras

PROVIDER: E-GEOD-31703 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ecological success of a group of Saccharomyces cerevisiae / Saccharomyces kudriavzevii hybrids in the Northern European wine making environment.

Project description:The aim of this project was to evaluate the ploidy of a S. cerevisiae *S. kudriavzevii hybrid in comparison to the lab strain S288C. Other wine yeast have been icluded in the project for the global analysis.

2012-02-09 | GSE31703 | GEO

Detection of S. cerevisiae fragments in the genome of S. bayanus strains with aCGH on Yeast_2 Affymetrix array.

Project description:The aim of this project was to detect S. cerevisiae fragments in the genome of S. bayanus Type strain CBS380 and pure line NBRC1948 (=IFO1948). The hybridization signal is compared to S. uvarum BBS7001 whose genome has been sequenced and in which no S. cerevisiae sequence has been detected. Comparison CBS380 (2 arrays) or IFO1948 (one array) to CBS 7001 (2 arrays).

2011-06-30 | E-GEOD-28225 | biostudies-arrayexpress

Molecular Analysis of the Genes Involved in Aroma Synthesis in the Species S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum in Winemaking

Project description:This research work investigates the hybridization of the genes of Saccharomyces bayanus var. uvarum CECT 12600 and S. kudriavzevii IFO1802 employing S. cerevisiae microarrays. Two strains,one S. kudriavzevii and one S. bayanus var. uvarum were employed for the study. The S. cerevisiae strain S288C was used as control for microarray hybridizations. All experiments were performed using duplicate arrays, and Cy5-dCTP and Cy3-dCTP dye-swap assays were performed to reduce dye-specific bias.

2014-06-23 | E-GEOD-52446 | biostudies-arrayexpress

Ecological success of a group of Saccharomyces cerevisiae/Saccharomyces kudriavzevii hybrids in the northern european wine-making environment.

Project description:The hybrid nature of lager-brewing yeast strains has been known for 25 years; however, yeast hybrids have only recently been described in cider and wine fermentations. In this study, we characterized the hybrid genomes and the relatedness of the Eg8 industrial yeast strain and of 24 Saccharomyces cerevisiae/Saccharomyces kudriavzevii hybrid yeast strains used for wine making in France (Alsace), Germany, Hungary, and the United States. An array-based comparative genome hybridization (aCGH) profile of the Eg8 genome revealed a typical chimeric profile. Measurement of hybrids DNA content per cell by flow cytometry revealed multiple ploidy levels (2n, 3n, or 4n), and restriction fragment length polymorphism analysis of 22 genes indicated variable amounts of S. kudriavzevii genetic content in three representative strains. We developed microsatellite markers for S. kudriavzevii and used them to analyze the diversity of a population isolated from oaks in Ardèche (France). This analysis revealed new insights into the diversity of this species. We then analyzed the diversity of the wine hybrids for 12 S. cerevisiae and 7 S. kudriavzevii microsatellite loci and found that these strains are the products of multiple hybridization events between several S. cerevisiae wine yeast isolates and various S. kudriavzevii strains. The Eg8 lineage appeared remarkable, since it harbors strains found over a wide geographic area, and the interstrain divergence measured with a (??)(2) genetic distance indicates an ancient origin. These findings reflect the specific adaptations made by S. cerevisiae/S. kudriavzevii cryophilic hybrids to winery environments in cool climates.

| S-EPMC3346444 | biostudies-literature

Transcriptomics of cryophilic Saccharomyces kudriavzevii reveals the key role of gene translation efficiency in cold stress adaptations

2013-11-21 | GSE52545 | GEO

Transcription profiling by array of a natural hybrid between Saccharomyces cerevisiae and S. kudriavzevii under enological conditions

Project description:Genome-wide gene expression of a natural hybrid between Saccharomyces cerevisiae and S. kudriavzevii under enological condition

2011-12-28 | E-TABM-1227 | biostudies-arrayexpress

Genomic and transcriptomic analysis of aroma synthesis in two hybrids between Saccharomyces cerevisiae and S. kudriavzevii in winemaking conditions

Project description:This research work investigates the expression of the genes involved in flavor compound production in two hybrids between Saccharomyces cerevisiae and S. kudriavzevii under low (12°C) and moderate fermentation temperatures (28°C).

2015-09-05 | GSE30779 | GEO

Saccharomyces kudriavzevii strain:CR85

Project description:Saccharomyces kudriavzevii CR85

| PRJNA480800 | ENA

Saccharomyces kudriavzevii

Project description:Comparative genomics between S. cerevisiae and S. kudriavzevii.

| PRJEB31099 | ENA

Transcriptomics of cryophilic Saccharomyces kudriavzevii reveals the key role of gene translation efficiency in cold stress adaptations

Project description:Comparative genome-wide gene expression analysis between a wine yeast strain belonging to the species S. cerevisiae and the type strain from S. kudriavzevii IFO1802, a cryotolerant yeast, in natural must fermentations. RNA samples taken from wine fermentations at 12M-BM-:C and 28M-BM-:C at the beginning of exponential phase. Three biological replicates.

2013-11-21 | E-GEOD-52545 | biostudies-arrayexpress

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