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AhNAS2 suppression in Arabidopsis halleri roots


ABSTRACT: The model Zn hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis gene expression. Suppression of AhNAS2 expression by RNAi resulted in strongly reduced root NA accumulation and a concomitant decrease in root-to-shoot-translocation of Zn but not of other micronutrients. Few secondary effects on the root transcriptome were detected by microarray analysis. Vegetatively propagated clones of A. halleri wildtype plants and a line with strong suppression of AhNAS2 were grown hydroponically for weeks in two different media (control Hoagland and 10 µM Zn2+ added). Roots were harvested and analyzed. Three plants were pooled for one sample. Three independent experiments were performed.

ORGANISM(S): Arabidopsis halleri ssp halleri

SUBMITTER: Stephan Clemens 

PROVIDER: E-GEOD-31778 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Elevated nicotianamine levels in Arabidopsis halleri roots play a key role in zinc hyperaccumulation.

Deinlein Ulrich U   Weber Michael M   Schmidt Holger H   Rensch Stefan S   Trampczynska Aleksandra A   Hansen Thomas H TH   Husted Søren S   Schjoerring Jan K JK   Talke Ina N IN   Krämer Ute U   Clemens Stephan S  

The Plant cell 20120228 2


Zn deficiency is among the leading health risk factors in developing countries. Breeding of Zn-enriched crops is expected to be facilitated by molecular dissection of plant Zn hyperaccumulation (i.e., the ability of certain plants to accumulate Zn to levels >100-fold higher than normal plants). The model hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to nonaccumulators among a core of alterations in metal homeostasis  ...[more]

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