Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

AhNAS2 suppression in Arabidopsis halleri roots


ABSTRACT: The model Zn hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis gene expression. Suppression of AhNAS2 expression by RNAi resulted in strongly reduced root NA accumulation and a concomitant decrease in root-to-shoot-translocation of Zn but not of other micronutrients. Few secondary effects on the root transcriptome were detected by microarray analysis. Vegetatively propagated clones of A. halleri wildtype plants and a line with strong suppression of AhNAS2 were grown hydroponically for weeks in two different media (control Hoagland and 10 µM Zn2+ added). Roots were harvested and analyzed. Three plants were pooled for one sample. Three independent experiments were performed.

ORGANISM(S): Arabidopsis halleri ssp halleri

SUBMITTER: Stephan Clemens 

PROVIDER: E-GEOD-31778 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Elevated nicotianamine levels in Arabidopsis halleri roots play a key role in zinc hyperaccumulation.

Deinlein Ulrich U   Weber Michael M   Schmidt Holger H   Rensch Stefan S   Trampczynska Aleksandra A   Hansen Thomas H TH   Husted Søren S   Schjoerring Jan K JK   Talke Ina N IN   Krämer Ute U   Clemens Stephan S  

The Plant cell 20120228 2


Zn deficiency is among the leading health risk factors in developing countries. Breeding of Zn-enriched crops is expected to be facilitated by molecular dissection of plant Zn hyperaccumulation (i.e., the ability of certain plants to accumulate Zn to levels >100-fold higher than normal plants). The model hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to nonaccumulators among a core of alterations in metal homeostasis  ...[more]

Similar Datasets

2011-12-31 | GSE31778 | GEO
2022-11-21 | E-NASC-44 | biostudies-arrayexpress
2007-01-08 | GSE5738 | GEO
2007-01-08 | GSE5613 | GEO
2008-06-14 | E-GEOD-5738 | biostudies-arrayexpress
2008-06-13 | E-GEOD-5613 | biostudies-arrayexpress
2022-03-02 | PXD026953 | Pride
2011-05-13 | E-GEOD-29236 | biostudies-arrayexpress
2021-04-21 | GSE168659 | GEO
2020-01-09 | PXD014578 | Pride