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Expression data from Ralstonia solanacearum-inoculated tomato stem

ABSTRACT: Bacterial wilt caused by Ralstonia solanacearum is a lethal, soil-borne disease of tomato. Control of the disease with chemicals and crop rotation is insufficient, because the pathogen is particularly well adapted for surviving in the soil and rhizosphere. Therefore, cultivar resistance is the most effective means for controlling bacterial wilt, but the molecular mechanisms of resistance responses remain unclear. We used microarrays to obtain the characteristics of the gene expression changes that are induced by R. solanacearum infection in resistant cultivar LS-89 and susceptible cultivar Ponderosa. Seedlings of LS-89 and Ponderosa at the five to six leaf-stage were inoculated on their stems just above the cotyledon by cutting to one-third the stem depth with a knife, adding a drop of bacterial suspension (1e+6 cfu/ml of R. solanacearum strain 8107S) or distilled water to the opening, and then clipping the wound site to avoid bending. Inoculated plants were grown in a growth chamber at 30ÂºC under 30,000 lux light intensity for 12 h a day. At 1dpi, stems were sampled by dissecting 5 mm long sections from 5 mm below the inoculation site. For each hybridization, RNA from 15 plants was used. Three biological replicates of microarray analysis were performed.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Takeaki Ishihara

PROVIDER: E-GEOD-31807 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from Ralstonia solanacearum-inoculated tomato stem

Project description:Bacterial wilt caused by Ralstonia solanacearum is a lethal, soil-borne disease of tomato. Control of the disease with chemicals and crop rotation is insufficient, because the pathogen is particularly well adapted for surviving in the soil and rhizosphere. Therefore, cultivar resistance is the most effective means for controlling bacterial wilt, but the molecular mechanisms of resistance responses remain unclear. We used microarrays to obtain the characteristics of the gene expression changes that are induced by R. solanacearum infection in resistant cultivar LS-89 and susceptible cultivar Ponderosa.

2012-10-06 | GSE31807 | GEO

Gene expression analysis of Mulberry root after inoculation with Ralstonia solanacearum.

Project description:Bacterial wilt, caused by the soil-borne bacterium Ralstonia solanacearum, is a lethal disease of mulberry, but the molecular mechanisms of the host resistance responses to R. solanacearum remain unclear. In order to better understand molecular resistance mechanisms to R. solanacearum in mulberry, we set out to define the changes in gene expression of resistance and susceptible mulberry cultivars after inoculation with R. solanacearum. Susceptible cultivar YSD10, resistance cultivar KQ10 and YS283 were inoculation with R. solanacearum, mulberry root samples were collected at 1 dpi and non-treated control in all cultivars. Then we performed RNA-Seq analyses on all mulberry root samples using Illumina HiSeq 2000.

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Transcriptom analysis of the interaction Medicago truncatula – Ralstonia solanacearum

Project description:affy_ralstonia_medicago - Ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease. Its infection process was studied with an in vitro inoculation procedure on intact roots of Medicago truncatula. The pathosystem involved susceptible A17 and resistant F83005.5 M truncatula lines infected with the pathogenic strain GMI1000. The mutant A17 line, Sickle, which showed a resistant phenotype was also part of the experiment. To identify host signaling pathway triggered by R. solanacearum infection with a focus on the involvment of ethylene, we used the Medicago Affymetrix array to monitore the expression profiles and the molecular process associated with initial symptoms development (12hpi) and colonization (72hpi). In order to maximize chances to observe differential gene expression, RNA samples were extracted from the root infection zone (root tips) -Three Medicago truncatula lines, A17, F83005.5 and sickle were inoculated with GMI1000 Ralstonai solanacearum strain (107 cfu/ml). RNA were extracted from root extremities (1 cm above the root tip) at time 0, 12h and 72h post inoculation. Three biological repeats were conducted normal vs disease comparison, time course, 27 arrays - Medicago

2011-09-09 | E-GEOD-18473 | biostudies-arrayexpress

Master transcription factors of plant immunity are targeted by a Ralstonia solanacearum effector

Project description:We found the Type III effector protein RipAB could suppress multiple plant immune responses and is important for the virulence of bacterial wilt pathogen Ralstonia solanacearum.

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Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

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Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C.

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Global transcriptome and targeted metabolite analyses of roots reveal different defense mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

Project description:The causal agent of bacterial wilt is Ralstonia solanacearum (Rs) with an unusually wide host range including potato. Bacterial wilt is the most destructive bacterial disease of plants that seriously reduce crop yield word-wide. Control of Rs through resistance breeding and biotechnology is considered to be very important and necessary. Rs resistance has been reported in a few potato cultivars and hybrids. Identification of defense mechanism underlying resistance is a prerequisite of biotechnological approaches. In this study, two resistant cultivars, ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR), and a sensitive cultivar, ‘Désirée’, were analysed and compared to each other at molecular level before-, and a few days after Rs infection. The transcriptome analysis of roots revealed up-regulation of chitin interaction and cell wall related genes in CR and DES. The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only CR. Microscopic analysis detected lignification over the whole stele in CR roots. In CG roots, concentrations of chlorogenic acid and quercetin derivatives were highly increased upon Rs infection. A characteristic increase at the transcript level of MAP kinase signalling pathway genes and concentrations of jasmonic-, salicylic-, abscisic and indoleacetic acid was detected in DES roots. These results indicate a different mechanism of defence both in the two tested resistant and the sensitive potato cultivar.

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Holliday junction resolvase RuvC targets biofilm eDNA to facilitate pathogen spread in plants

Project description:Biofilm lifestyle is critical for bacterial pathogens to colonize and protect themselves from host immunity and antimicrobial chemicals in plants and animals. The formation and regulation mechanism of phytobacterial biofilm are still obscure. Here, we found that Ralstonia solanacearum Resistance to ultraviolet C (RuvC) is highly abundant in biofilm and positively regulates pathogenicity by governing systemic movement in tomato xylem. RuvC protein accumulates at the later stage of biofilm and specifically targets the Holliday junction (HJ) like structures to disrupt biofilm extracellular DNA (eDNA) lattice, thus facilitating biofilm dispersal. Recombinant RuvC protein can resolve extracellular HJ prevent bacterial biofilm formation. Heterologous expression of R. solanacearum or Xanthomonas oryzae pv. oryzae RuvC with plant secretion signal in tomato or rice confers resistance to bacterial wilt or bacterial blight disease, respectively. Plant chloroplast localized HJ resolvase monokaryotic chloroplast 1 (MOC1) which is structural similar to bacterial RuvC shows a strong inhibit effect on bacterial biofilm formation. Re-localization of SlMOC1 to apoplast in tomato roots leads to increase resistance to bacterial wilt. Our novel finding reveals a critical pathogenesis mechanism of R. solanacearum and provides an efficient biotechnology strategy to improve plant resistance to bacteria vascular disease.

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Expression data from Arabidopsis thaliana Col-0 challenged with Ralstonia solanacearum race 4

Project description:Bacterial wilt caused by Ralstonia solanacearum is a serious seed/soil borne disease that causes severe yield and quality losses in many plants. In order to understand the change in genome expression of inculated plants, microarray analysis were performed.

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Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain GMI1000 at 20°C and 28°C in culture and in planta. The tropical strain GMI1000 cannot wilt tomato plants at 20°C although it can cause full-blown disease at 28°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain GMI1000 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain GMI1000 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 5061 genes from Ralstonia solanacearum strain GMI1000 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

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