Unknown,Transcriptomics,Genomics,Proteomics

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Targeted ErbB3 loss in mammary organoids harvested from ErbB3 DOX-KO mice +/- DOX treatment


ABSTRACT: Mammary organoids harvested from ErbB3 DOX-KO mice, which utilize MMTV-Cre transgene expression in the LE to cause genomic recombination at floxed ErbB3 alleles in ErbB3FL/FL were cultured in the presence or absence of doxycycline to induce ErbB3 loss. The gene expression shift following DOX-induced ErbB3 loss in the 3D organoids was examined by microarray. Gene expression patterns were interrogated in mammary organoids from ErbB3 inducible-knockout mice cultured in the presence of absence of doxycycline. Three biological replicates of the experiment were performed, resulting in a total of 6 samples (3 treatment, 3 control).

ORGANISM(S): Mus musculus

SUBMITTER: Justin Balko 

PROVIDER: E-GEOD-32129 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The receptor tyrosine kinase ErbB3 maintains the balance between luminal and basal breast epithelium.

Balko Justin M JM   Miller Todd W TW   Morrison Meghan M MM   Hutchinson Katherine K   Young Christian C   Rinehart Cammie C   Sánchez Violeta V   Jee David D   Polyak Kornelia K   Prat Aleix A   Perou Charles M CM   Arteaga Carlos L CL   Cook Rebecca S RS  

Proceedings of the National Academy of Sciences of the United States of America 20111216 1


ErbB3 harbors weak kinase activity, but strongly activates downstream phosphatidylinositol 3-kinase/Akt signaling through heterodimerization with and activation by other ErbB receptor tyrosine kinases. We report here that ErbB3 loss in the luminal mammary epithelium of mice impaired Akt and MAPK signaling and reduced luminal cell proliferation and survival. ERBB3 mRNA expression levels were highest in luminal mammary populations and lowest in basal cell/stem cell populations. ErbB3 loss in mamma  ...[more]

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