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Retinal gene expression of low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice

ABSTRACT: Retinal gene expression was assessed from postnatal day (P) 8 old low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice with a Illumina mouse-WG6 expression BeadChip. RNA was extracted from the retinas of P8 Lrp5 knockout and wild type mice (n=3 each group). Total RNA (1ug each) were reverse transcribed, followed by a single in vitro transcription amplification to incorporate biotin-labeled nucleotide, and subsequent hybridization and staining with strepatavidin-Cy3 according to the manufacturer’s instructions.

ORGANISM(S): Mus musculus

SUBMITTER: Jing Chen

PROVIDER: E-GEOD-32145 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Retinal expression of Wnt-pathway mediated genes in low-density lipoprotein receptor-related protein 5 (Lrp5) knockout mice.

Chen Jing J Stahl Andreas A Krah Nathan M NM Seaward Molly R MR Joyal Jean-Sebastian JS Juan Aimee M AM Hatton Colman J CJ Aderman Christopher M CM Dennison Roberta J RJ Willett Keirnan L KL Sapieha Przemyslaw P Smith Lois E H LE

PloS one 20120117 1

Mutations in low-density lipoprotein receptor-related protein 5 (Lrp5) impair retinal angiogenesis in patients with familial exudative vitreoretinopathy (FEVR), a rare type of blinding vascular eye disease. The defective retinal vasculature phenotype in human FEVR patients is recapitulated in Lrp5 knockout (Lrp5(-/-)) mouse with delayed and incomplete development of retinal vessels. In this study we examined gene expression changes in the developing Lrp5(-/-) mouse retina to gain insight into th ...[more]

PMID: 22272305

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