Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcrioptome analysis of human primary macrophages stimulated by bacterial endotoxin lipopolysaccharide (LPS), and (1,3)-b-glucans, curdlan and glucan from bakerM-BM-4s yeast (GBY)

ABSTRACT: We performed gene expression microarray experiments to compare the global transcriptional response induced by b-glucans and LPS with their secretomes. We identified 1683, 767 and 1447 genes with over two-fold increase or decrease in LPS-, curdlan- or GBY-stimulated macrophages, respectively. We show that both LPS and b-glucan induces significant gene expression changes in macrophages, but only b-glucans activate a robust protein secretion. The gene expression of human primary macrophage cells was measured at 6 hours after exposure the cells to 1 M-BM-5g LPS or 10 M-BM-5g Curdlan or 100 M-BM-5g GBY (glucan from bakerM-BM-4s yeast), also 0-group was included. Three independent experiments were performed using three different donors for each experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Tiina M-CM-^Vhman

PROVIDER: E-GEOD-32282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dectin-1 pathway activates robust autophagy-dependent unconventional protein secretion in human macrophages.

Öhman Tiina T Teirilä Laura L Lahesmaa-Korpinen Anna-Maria AM Cypryk Wojciech W Veckman Ville V Saijo Shinobu S Wolff Henrik H Hautaniemi Sampsa S Nyman Tuula A TA Matikainen Sampsa S

Journal of immunology (Baltimore, Md. : 1950) 20140507 12

Dectin-1 is a membrane-bound pattern recognition receptor for β-glucans, which are the main constituents of fungal cell walls. Detection of β-glucans by dectin-1 triggers an effective innate immune response. In this study, we have used a systems biology approach to provide the first comprehensive characterization of the secretome and associated intracellular signaling pathways involved in activation of dectin-1/Syk in human macrophages. Transcriptome and secretome analysis revealed that the dect ...[more]

PMID: 24808366

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Project description:A comprehensive in vitro assessment of two commercial metal oxide nanoparticles, TiO2 and ZnO, was performed using human monocyte-derived macrophages (HMDM), monocyte-derived dendritic cells (MDDC), and T cell leukemia-derived cell line (Jurkat). TiO2 nanoparticles were found to be non-toxic whereas ZnO nanoparticles caused dose-dependent cell death. Subsequently, global gene expression profiling was performed to identify signaling pathways underlying the cytotoxicity caused by ZnO nanoparticles. Analysis was done with doses, 1M-BM-5g/ml and 10M-BM-5g/ml after 6 and 24 hours of exposure. Interestingly, 2703 genes were significantly differentially expressed in HMDM upon exposure to 10M-BM-5g/ml ZnO nanoparticles, while in MDDCs only 12 genes were affected. In Jurkat cells, 980 genes were differentially expressed. It is noteworthy that the gene expression of metallothioneins was upregulated in all the three cell types. In addition to the common ZnO-inducible changes, a notable proportion of the genes were regulated in a cell type-specific manner. Using a panel of ZnO nanoparticles, we obtained an additional support that the cellular response to ZnO nanoparticles is caused by particle dissolution. Gene ontology analysis revealed that the top biological processes disturbed in HMDM and Jurkat cells were regulating cell death and growth. In addition, genes controlling immune system development were affected. Bioinformatics assessment showed that the top human disease category associated with ZnO-responsive genes in both HMDM and Jurkat cells was cancer. Overall, the study revealed novel genes and pathways for mediating ZnO nanoparticle-induced toxicity and demonstrated the value of assessing nanoparticle responses through combined transcriptomics and bioinformatics approach. Altogether 90 samples were analyzed originating from three biological replicates of HMDM, MDDC or Jurkat cells containg untreated control cells, ZnO or TiO2 treated cells with doses 1M-BM-5g/ml and 10M-BM-5g/ml. The sample timepoints were 6 hours and 24 hours.

Dataset Information

Transcrioptome analysis of human primary macrophages stimulated by bacterial endotoxin lipopolysaccharide (LPS), and (1,3)-b-glucans, curdlan and glucan from bakerM-BM-4s yeast (GBY)

Publications

Dectin-1 pathway activates robust autophagy-dependent unconventional protein secretion in human macrophages.

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