Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Jason Gertz
PROVIDER: E-GEOD-32307 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Gertz Jason J Varley Katherine E KE Davis Nicholas S NS Baas Bradley J BJ Goryshin Igor Y IY Vaidyanathan Ramesh R Kuersten Scott S Myers Richard M RM
Genome research 20111129 1
RNA-seq has been widely adopted as a gene-expression measurement tool due to the detail, resolution, and sensitivity of transcript characterization that the technique provides. Here we present two transposon-based methods that efficiently construct high-quality RNA-seq libraries. We first describe a method that creates RNA-seq libraries for Illumina sequencing from double-stranded cDNA with only two enzymatic reactions. We generated high-quality RNA-seq libraries from as little as 10 pg of mRNA ...[more]