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Transcription profiling of mouse Sca1+ primary myospheres isolated from Balb/c animals

ABSTRACT: Cells were isolated from muscle (hind leg) of Balb/c mouse (male). Then cells were enriched at FACS by using Sca1 antigen. Cells were grown 4 days in NSC medium, passaged and RNA isolated. Cells were at passage 1. Experiment Overall Design: this experiment include 1 samples and 6 replicates

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-3235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sca1+ primary myospheres

Project description:Cells were isolated from muscle (hind leg) of Balb/c mouse (male). Then cells were enriched at FACS by using Sca1 antigen. Cells were grown 4 days in NSC medium, passaged and RNA isolated. Cells were at passage 1. Keywords: other

2005-10-06 | GSE3235 | GEO

Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

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Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

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HDAC inhibitors promote the pro-myogenic activity of Sca1-positive interstitial cells in regenerating dystrophic muscles

Project description:We report here on the identification and functional characterization of Sca1-positive muscle interstitial cells that contribute to regeneration or fibroadipogenic degeneration of dystrophic muscles and mediate the beneficial effects of HDAC inhibitors (HDACi) in mdx mice. We found that the phenotype adopted by these cells and their biological activity are influenced by changes in muscle environment. While Sca1-positive muscle interstitial cells from healthy muscles spontaneously adopt a fibro-adipogenic phenotype, Sca1-positive muscle interstitial cells isolated from dystrophic muscles of young mdx mice show a latent myogenic phenotype that is implemented by the exposure to HDACi. Co-culture assays in vitro and co-transplantation experiments in vivo demonstrate that HDACi also improve Sca1-positive muscle interstitial cell ability to enhance the differentiation potential of adjacent satellite cells. Importantly, HDACi-induced myogenic phenotype and pro-regeneration activity were not observed in Sca1-positive muscle interstitial cells isolated from muscles of old mdx mice. The different phenotype of Sca1-positive muscle interstitial cells from mdx mice at different stages of disease progression correlated with the stage-dependent beneficial effect of HDACi, which were effective only at early stages of disease. Transplantation of Sca1-positive muscle interstitial cells isolated from regenerating young muscles into muscles of old mdx mice restored HDACi ability to increase myofiber size. These results indicate that Sca1-positive muscle interstitial cells are key cellular determinants of disease progression and mediate the beneficial effect of HDACi in a mouse model of muscular dystrophy.

2012-04-01 | GSE34843 | GEO

Chromatin accessibilty profiling of SCA1+ and SCA1- lineage traced VSMCs

Project description:VSMCs expressing SCA1 have increased proliferative capacity (Dobnikar et al, 2018; Worssam et al, 2022; Pan et al, 2020). We therefore, mapped chromatin accessibility changes using bulk ATAC-seq for SCA1+ and SCA1- lineage traced VSMCs.

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Chromatin accessibilty profiling of SCA1+ and SCA1- lineage traced VSMCs

Project description:Chromatin accessibilty profiling of SCA1+ and SCA1- lineage traced VSMCs

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Modulation of ATXN1 S776 phosphorylation reveals the importance of allele-specific targeting in SCA1

Project description:RNA was isolated from mouse cerebellum at 6 weeks of age in 5 different gentoypes; wild-type (WT), Atxn1_154Q/2Q (SCA1), Atxn1_154Q[S776A]/2Q (SCA1 S776A), Atxn1_154Q[S776A]/2Q[S776A] (S776A Double) and Atxn1_2Q[S776A]/2Q[S776A] (homo). After RNA isolation, RNA-seq was performed and gene expression profiles were compared between WT, SCA1, and the S776A mutants. The goal was to determine if mutating the phosphorylation site S776 in the context of spinocerebellar ataxia type 1 (SCA1) is protective.

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HDAC inhibitors promote the pro-myogenic activity of Sca1-positive interstitial cells in regenerating dystrophic muscles

2012-03-31 | E-GEOD-34843 | biostudies-arrayexpress

Single cell transcriptional profiling of native, transplanted, or organoid SCA1+ and SCA1- lung epithelial cells

Project description:The study examined the ability of SCA1+ and SCA1- lung epithelial organoids to be transplanted and retained by donor mice.

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Gene expression profiling of CD45+/Sca1+ cells isolated from the bone marrow and the muscle

Project description:The BM-derived CD45+/Sca1+ cells are haematopoietic stem/progenitor cells that have the ability to circulate and migrate and engraft to the muscle tissue, and therefore they are of particular interest. Notably, these cells retain their haematopoietic potential, as revealed both by in vitro and in vivo assays; but they also acquire myogenic potential, as shown by their ability to participate in muscle regeneration. Whether, this latter remarkable ability is the result of the reprogramming of the BM-CD45+/Sca1+ cells and the activation of a myogenic molecular program within these cells, remains controversial. This study aims to clarify this aspect of the process, investigating the role of the muscle microenviroment and key myogenic transcription factors. Keywords: CD45+/Sca1+ cells, BM, muscle CD45+/Sca1+ cells isolated from the BM or the muscle were processed fresh and their RNA was extracted. Moreover, CD45+/Sca1+ cells isolated from the muscle of BM transplanted or untransplanted mice after injury with Cardiotoxin were processed fresh and their RNA was extracted.

2010-04-05 | E-GEOD-9296 | biostudies-arrayexpress

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