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Differential Expressions of miRNAs in resting and activated human in vitro-differentiated NK cells

ABSTRACT: To investigate miRNA expressions of miRNA upon activation. In vitro-differentiated human NK cells were freshly incubated in the presence of IL15 after 24h deprivation of IL-15. The cells were harvested at the times (0h, resting-Sample 1 and 2; 6h, activated-Sample 3 and 4) and analyzed by microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Tae-Don Kim

PROVIDER: E-GEOD-32411 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human microRNA-27a* targets Prf1 and GzmB expression to regulate NK-cell cytotoxicity.

Kim Tae-Don TD Lee Su Ui SU Yun Sohyun S Sun Hu-Nan HN Lee Suk Hyung SH Kim Jae Wha JW Kim Hwan Mook HM Park Song-Kyu SK Lee Chang Woo CW Yoon Suk Ran SR Greenberg Philip D PD Choi Inpyo I

Blood 20110929 20

Perforin (Prf1) and granzyme B (GzmB) are essential effector molecules for natural killer (NK)-cell cytotoxicity, but how Prf1 and GzmB expression is regulated during arming of NK cells is poorly defined. We show that human microRNA (miR)-27a* is a negative regulator of NK-cell cytotoxicity by silencing Prf1 and GzmB expression. Human miR-27a* specifically bound to the 3' untranslated regions of Prf1 and GzmB, down-regulating expression in both resting and activated NK cells, and it functioned a ...[more]

PMID: 21960590

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