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The genetics and regulatory logic of alternative polyadenylation in human lymphoblastoid cells

ABSTRACT: We set out to investigate genomic mechanisms by which alternative polyadenylation impacts gene expression and its variation across genetically distinct human individuals. We used 3’-end RNA-seq to maximize genome coverage and precision of transcript end positions, and to measure quantitative expression levels of transcript forms. The results shed light on the architecture of transcript ends and regulatory elements in human 3’ UTRs and the principles of genetic variation in 3’ length form usage. Survey of transcript 3' end positions in B-lymphocyte cell lines derived from six human individuals (two biological replicates each)

ORGANISM(S): Homo sapiens

SUBMITTER: Oh Kyu Yoon

PROVIDER: E-GEOD-33154 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetics and regulatory impact of alternative polyadenylation in human B-lymphoblastoid cells.

Yoon Oh Kyu OK Hsu Tiffany Y TY Im Joo Hyun JH Brem Rachel B RB

PLoS genetics 20120816 8

Gene expression varies widely between individuals of a population, and regulatory change can underlie phenotypes of evolutionary and biomedical relevance. A key question in the field is how DNA sequence variants impact gene expression, with most mechanistic studies to date focused on the effects of genetic change on regulatory regions upstream of protein-coding sequence. By contrast, the role of RNA 3'-end processing in regulatory variation remains largely unknown, owing in part to the challenge ...[more]

PMID: 22916029

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