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Transcription profiling of different regions of mouse brain to study adult mouse gene expression patterns in common strains.

ABSTRACT: Adult mouse gene expression patterns in common strains. Experiment Overall Design: six mouse strains and seven brain regions were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: Carrolee Barlow

PROVIDER: E-GEOD-3327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice.

Hovatta Iiris I Tennant Richard S RS Helton Robert R Marr Robert A RA Singer Oded O Redwine Jeffrey M JM Ellison Julie A JA Schadt Eric E EE Verma Inder M IM Lockhart David J DJ Barlow Carrolee C

Nature 20051023 7068

Anxiety and fear are normal emotional responses to threatening situations. In human anxiety disorders--such as panic disorder, obsessive-compulsive disorder, post-traumatic stress disorder, social phobia, specific phobias and generalized anxiety disorder--these responses are exaggerated. The molecular mechanisms involved in the regulation of normal and pathological anxiety are mostly unknown. However, the availability of different inbred strains of mice offers an excellent model system in which ...[more]

PMID: 16244648

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Transcription profiling of mouse brain regions (bed nucleus of the stria terminalis, hippocampus, hypothalamus, periaqueductal gray, and pituitary gland) in six mouse strains (129S6/SvEvTac, A/J, C57BL/6J, C3H/HeJ, DBA/2J, and FVB/NJ)

Project description:Gene expression patterns were determined from five brain regions (bed nucleus of the stria terminalis, hippocampus, hypothalamus, periaqueductal gray, and pituitary gland) in six mouse strains (129S6/SvEvTac, A/J, C57BL/6J, C3H/HeJ, DBA/2J, and FVB/NJ). At least two replicate samples per brain region/strain were analyzed using Affymetrix Mouse Genome 430 2.0 arrays. Experiment Overall Design: six mouse strains and five brain regions were analyzed

2009-09-16 | E-GEOD-4734 | biostudies-arrayexpress

Venomics of the Central-European Myrmicine Ants Myrmica rubra and Myrmica ruginodis

Project description:Animal venoms are a rich source of novel biomolecules with tremendous potential in medicine and agriculture. Ants represent one of the most species-rich lineages of venomous animals. However, only a fraction of their biodiversity has been studied so far. Here, we investigated the venom compositions from Myrmica rubra and Myrmica ruginodis, two members of the Myrmicinae subfamily of ants. We applied a proteo-transcriptomics based venomics workflow. Our analysis revealed that venoms of both species are composed of several protein classes, such as venom serine protease, cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 proteins (CAP), Kunitz-type serine protease inhibitors or venom acid phosphatase. Several protein classes identified are known venom allergens, and for the first time we detected phospholipase A1 in the venom of M. ruginodis. We also identified two novel toxins of the epidermal growth factor (EGF) family in its venom proteome and an array of additional EGF-like toxins in venom gland transcriptomes of both species. They display similarity to known toxins from the related myrmecine Manica rubida and the Australian red bulldog ant Myrmecia gullosa of the Myrmeciinae subfamily and may serve as nociceptive weapons in defensive scenarios. Our work suggests that the venoms of M. rubra and M. ruginodis contain many high molecular proteins and enzymes with putatively cell damaging functions. Nevertheless, the presence of EGF-like toxins underpins that myrmicine ants also recruited smaller peptide components into their venom arsenal. Although little is known about the bioactivity and function of these EGF-like toxins, their presence in Myrmecinae and Myrmeciinae suggests that they play an important role for the venom systems of Formicoidea. Our work adds to the emerging picture of ant venoms as a source for novel biomolecules. This underlines the importance to incorporate such taxa in future venom bioprospecting programs.

2022-06-09 | PXD033537 | Pride

Transcription profiling of rat lumbar spinal cord following ventral root avulsion in two inbred strains to investigate susceptibility to neurodegeneration

Project description:Gene expression profiling in rat lumbar spinal cord following ventral root avulsion in the two inbred rat strains.

2006-08-15 | E-MEXP-671 | biostudies-arrayexpress

Identification of early neurodegenerative pathways in progressive multiple sclerosis

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2023-08-17 | E-MTAB-13083 | biostudies-arrayexpress

Hypothalamic gene expression profile indicates a reduction in G-protein signaling in the wfs1 mutant mice

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Gene expression profiling of vasoregression in the retina

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2011-03-15 | E-GEOD-20967 | biostudies-arrayexpress

Comprehensive proteome of the adult neural stem cell niche identifies key regulators of adult neurogenesis

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Gene expression profiling of temporal lobes of wfs1 deficient mice

Project description:Aim of present study was to describe the changes induced deletion of the Wfs1 gene in the temporal lobe of mice. Mutant mice were backcrossed to two different genomic backgrounds in order to exclude confounding foreign genomic background influence. Samples from temporal lobes were analyzed by using Affymetrix Genechips, expression profiles were functionally annotated by using GSEA and Ingenuity Pathway Analysis. We found that Wfs1 mutant mice are significantly smaller (20.9 +/- 1.6 g) than their wild-type counterparts (31.0 +/- 0.6 g, p < 0.0001). Interestingly, genechip analysis identified growth hormone transcripts up-regulated and functional analysis found appropriate pathways activated. Moreover, we found significant increase in the level of IGF1 in the plasma of wfs1 mutant mice. Taken together, wfs1 mutation induces growth retardation whereas the growth hormone pathway is activated. Further studies are needed to describe biochemical and molecular details of the growth hormone axis in the wfs1 mutant mice. 37 samples, two genotypes (wild type and mutant), in two different genetic background (C57B6 and 129S6)

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