Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

CcpA, a Pleiotropic Key Regulator in Butanol-producing Clostridium acetobutylicum ATCC 824


ABSTRACT: Clostridium acetobutylicum is a typical bacterium of major importance to industrial butanol production. In order to dissect the regulatory network pertaining to the industrial application of this bacterium, catabolite control protein A (CcpA) was investigated for its global function by DNA microarray.It showed that CcpA of C. acetobutylicum controls hundreds of genes, not only carbon metabolism, but also solvent production and sporulation in the life cycle.The results here demonstrated that CcpA is an important pleiotropic regulator related to some specific physiological and biochemical process in butanol-producing C. acetobutylicum. In order to enable a global understanding of the regulatory roles of CcpA when fermenting mixed sugars, which is of great significance in utilization of lignocellulosic hydrolysates, D-glucose plus D-xylose were used as the carbon sources in fermentation for microarray analysis. Microarray analysis was performed at four time points:the time point M and L were chosen both in acidogenic phase, while the time point T and S were chosen in shift phase (from acidogenesis to solventogenesis) and solventogenic phase, respectively.One-color microarray assays were performed.Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). The ratio of transcript level between wildtype and mutant can been achieved using the formula: 2^(value of wildtype)/2^(value of ccpA mutant).

ORGANISM(S): Clostridium acetobutylicum

SUBMITTER: Cong Ren 

PROVIDER: E-GEOD-33364 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum.

Ren Cong C   Gu Yang Y   Wu Yan Y   Zhang Weiwen W   Yang Chen C   Yang Sheng S   Jiang Weihong W  

BMC genomics 20120730


<h4>Background</h4>Clostridium acetobutylicum has been used to produce butanol in industry. Catabolite control protein A (CcpA), known to mediate carbon catabolite repression (CCR) in low GC gram-positive bacteria, has been identified and characterized in C. acetobutylicum by our previous work (Ren, C. et al. 2010, Metab Eng 12:446-54). To further dissect its regulatory function in C. acetobutylicum, CcpA was investigated using DNA microarray followed by phenotypic, genetic and biochemical valid  ...[more]

Similar Datasets

2012-08-30 | GSE33364 | GEO
2015-05-15 | E-GEOD-68899 | biostudies-arrayexpress
2015-05-15 | E-GEOD-68900 | biostudies-arrayexpress
2021-07-30 | PXD021161 | Pride
2012-01-30 | E-MEXP-3571 | biostudies-arrayexpress
2017-09-27 | E-MTAB-6019 | biostudies-arrayexpress
2012-04-30 | E-MEXP-3264 | biostudies-arrayexpress
2012-04-30 | E-MEXP-3265 | biostudies-arrayexpress
2015-09-09 | E-GEOD-72765 | biostudies-arrayexpress
2010-11-10 | E-MEXP-2503 | biostudies-arrayexpress