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Cross-species Detection in Barley1 GeneChip Array

ABSTRACT: This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB1 at PLEXdb.] species: Barley(2-replications); species: Wheat(2-replications); species: Oat(2-replications); species: Sorghum(2-replications); species: Corn(2-replications); species: Rice(2-replications)

ORGANISM(S): Triticum aestivum

SUBMITTER: Rico Caldo

PROVIDER: E-GEOD-33391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A new resource for cereal genomics: 22K barley GeneChip comes of age.

Close Timothy J TJ Wanamaker Steve I SI Caldo Rico A RA Turner Stacy M SM Ashlock Daniel A DA Dickerson Julie A JA Wing Rod A RA Muehlbauer Gary J GJ Kleinhofs Andris A Wise Roger P RP

Plant physiology 20040301 3

In recent years, access to complete genomic sequences, coupled with rapidly accumulating data related to RNA and protein expression patterns, has made it possible to determine comprehensively how genes contribute to complex phenotypes. However, for major crop plants, publicly available, standard platforms for parallel expression analysis have been limited. We report the conception and design of the new publicly available, 22K Barley1 GeneChip probe array, a model for plants without a fully seque ...[more]

PMID: 15020760

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2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33400 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress

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Cross-species Detection in Barley1 GeneChip Array

Project description:This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB1 at PLEXdb.]

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Project description:Comparison of mRNA accumulation in segregating doubled haploid barley lines ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB21 at PLEXdb.] genetic line: SM004(3-replications); genetic line: SM007(3-replications); genetic line: SM012(3-replications); genetic line: SM013(3-replications); genetic line: SM022(3-replications); genetic line: SM024(3-replications); genetic line: SM027(3-replications); genetic line: SM041(3-replications); genetic line: SM043(3-replications); genetic line: SM044(3-replications); genetic line: SM046(3-replications); genetic line: SM061(3-replications); genetic line: SM063(3-replications); genetic line: SM073(3-replications); genetic line: SM074(3-replications); genetic line: SM079(3-replications); genetic line: SM085(3-replications); genetic line: SM088(3-replications); genetic line: SM089(3-replications); genetic line: SM116(3-replications); genetic line: SM130(3-replications); genetic line: SM135(3-replications); genetic line: SM136(3-replications); genetic line: SM140(3-replications); genetic line: SM141(3-replications); genetic line: SM146(3-replications); genetic line: SM152(3-replications); genetic line: SM155(3-replications); genetic line: SM160(3-replications); genetic line: SM167(3-replications); genetic line: SM169(3-replications); genetic line: SM173(3-replications); genetic line: SM177(3-replications); genetic line: SM184(3-replications); genetic line: SM200(3-replications); genetic line: Steptoe(3-replications); genetic line: Morex(3-replications)

2011-11-22 | E-GEOD-33394 | biostudies-arrayexpress

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2011-11-22 | E-GEOD-33398 | biostudies-arrayexpress

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