Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cross-species Detection in Barley1 GeneChip Array

ABSTRACT: This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB1 at PLEXdb.] species: Barley(2-replications); species: Wheat(2-replications); species: Oat(2-replications); species: Sorghum(2-replications); species: Corn(2-replications); species: Rice(2-replications)

ORGANISM(S): Triticum aestivum

SUBMITTER: Rico Caldo

PROVIDER: E-GEOD-33391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

A new resource for cereal genomics: 22K barley GeneChip comes of age.

Close Timothy J TJ Wanamaker Steve I SI Caldo Rico A RA Turner Stacy M SM Ashlock Daniel A DA Dickerson Julie A JA Wing Rod A RA Muehlbauer Gary J GJ Kleinhofs Andris A Wise Roger P RP

Plant physiology 20040301 3

In recent years, access to complete genomic sequences, coupled with rapidly accumulating data related to RNA and protein expression patterns, has made it possible to determine comprehensively how genes contribute to complex phenotypes. However, for major crop plants, publicly available, standard platforms for parallel expression analysis have been limited. We report the conception and design of the new publicly available, 22K Barley1 GeneChip probe array, a model for plants without a fully seque ...[more]

PMID: 15020760

Similar Datasets

Microarray of barley genes in wheat-barley chromosome addition lines

Project description:Transcript levels of barley genes were examined in the wheat-barley chromosome addition lines having one of six barley chromomes, 2H, 3H, 4H, 5H, 6H and 7H. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is BB8 at PLEXdb.] genotype: chr2H(3-replications); genotype: chr2HL(3-replications); genotype: chr3H(3-replications); genotype: chr4H(3-replications); genotype: chr5H(3-replications); genotype: chr6H(3-replications); genotype: chr6HL(3-replications); genotype: chr6HS(3-replications); genotype: chr7H(3-replications); genotype: Betzes(3-replications); genotype: Chinese Spring(3-replications)

2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines

Project description:We utilized the Barley1 Affymetrix GeneChip for comparative transcript analysis of Betzes barley, Chinese Spring wheat, and Chinese SpringM-bM-^@M-^SBetzes ditelosomic chromosome addition lines to physically map barley genes to their respective chromosome arm locations. We mapped barley genes to chromosome arms (1HS, 2HS, 2HL, 3HS, 3HL, 4HS, 4HL, 5HS, 5HL, 7HS, and 7HL) based on their transcript levels in the ditelosomic addition lines. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Hatice Bilgic. The equivalent experiment is BB55 at PLEXdb.] genotype: CS (Chinese Spring)(3-replications); genotype: Betzes(3-replications); genotype: 1HS(3-replications); genotype: 2HS(3-replications); genotype: 3HS(3-replications); genotype: 3HL(3-replications); genotype: 4HS(3-replications); genotype: 4HL(3-replications); genotype: 5HS(3-replications); genotype: 5HL(3-replications); genotype: 7HS(3-replications); genotype: 7HL(3-replications)

2011-11-22 | E-GEOD-33400 | biostudies-arrayexpress

Genotype-dependent gene expression in barley

Project description:Comparison of mRNA accumulation in the seedling leaves of 8 unstressed barley cultivars ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB20 at PLEXdb.] genotype: Morex(3-replications); genotype: Steptoe(3-replications); genotype: OWB REC(3-replications); genotype: OWB DOM(3-replications); genotype: Barke(3-replications); genotype: Haruna Nijo(3-replications); genotype: Golden Promise(3-replications); genotype: Optic(3-replications)

2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress

mlo5-mediated resistance responses in barley

Project description:Gene expression of a near-isogenic pair of barley lines differing in absence or presence of the mlo5 resistance gene in response to attack by barley powdery mildew (Bgh). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Achim Fischer. The equivalent experiment is BB7 at PLEXdb.] disease type: Control - genotype: Mlo(1-replications); disease type: Control - genotype: mlo5(1-replications); disease type: Treated (Pathogen Infection) - genotype: Mlo(1-replications); disease type: Treated (Pathogen Infection) - genotype: mlo5(1-replications)

2011-11-22 | E-GEOD-33406 | biostudies-arrayexpress

Expression analysis of gene silenced barley to identify the function of a novel LRR-RLK (leucine-rich repeat receptor-like kinase)

Project description:VIGS (virus-induced gene silencing) was used to silence a putative LRR-RLK, Affy ID Rbaal9i05_at, in barley. Expression patterns in leaves were compared between untreated control leaves, virus treated leaves, VIGS-Rbaal knockdown leaves and VIGS-Rbaal/PDS (phytoene desaturase) co-silenced leaves. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David L Parrott. The equivalent experiment is BB108 at PLEXdb.] treatment: Untreated Control(3-replications); treatment: Empty Virus (no inserted plant sequences)(3-replications); treatment: Rbaal VIGS(3-replications); treatment: Co-silencing (Rbaal + PDS)(3-replications)

2011-12-03 | E-GEOD-34102 | biostudies-arrayexpress

Structural and functional characterization of a winter malting barley

Project description:Transcriptome comparison of the winter malting barley '88Ab536' with the spring malting variety 'Morex' at two time points of the malting process: 'out of steeping' and '3 days of germination'. Three replicates of each genotype and time point were accomplished. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Maria Munoz-Amatriain. The equivalent experiment is BB76 at PLEXdb.] genotype: 88Ab536 - time: 0 days(3-replications); genotype: 88Ab536 - time: 3 days(3-replications); genotype: Morex - time: 0 days(3-replications); genotype: Morex - time: 3 days(3-replications)

2011-11-22 | E-GEOD-33842 | biostudies-arrayexpress

Cross-species Detection in Barley1 GeneChip Array

Project description:This study was conducted to evaluate the efficiency of cross-species detection in Barley1 GeneChip array. We hybridized cRNA derived from first leaves of barley green seedlings (as a control), as well as the same stage of seedling leaf from representative genotypes of wheat, oat, rice, maize, and sorghum. Ten to twenty seedlings for each species were harvested and pooled for RNA preparation, labeling, and hybridization. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB1 at PLEXdb.]

2011-11-22 | GSE33391 | GEO

Mapping translocation breakpoints using a wheat microarray

Project description:Five RNA samples were used. The RNA source material was from wheat cytogenetic stocks from Dr. Adam Lukaszewski, University of California, Riverside. The lines used for this study are Pavon, Pavon Dt1BL, 1RS.1BL, 1B+5 and T-1 wheat samples. whole seedling minus starchy endosperm. Equal portions of RNA from unstressed and 48 hr drought-stressed seedling were combined to make one RNA sample. The person who produced the RNA was Prasanna R. Bhat in the TJ Close lab at University of California, Riverside. Everything downstream of RNA production was done at the UC Irvine DNA and Protein Microarray Facility (Denis Heck, manager) by Sriti Misra. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Prasanna Bhat. The equivalent experiment is TA10 at PLEXdb.] genetic line: Pavon(2-replications); genetic line: Pavon Dt1BL(2-replications); genetic line: 1RS.1BL(2-replications); genetic line: T-1(2-replications); genetic line: 1B+5(2-replications)

2011-08-30 | E-GEOD-31754 | biostudies-arrayexpress

Genetics of gene expression in barley

Project description:Comparison of mRNA accumulation in segregating doubled haploid barley lines ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB21 at PLEXdb.] genetic line: SM004(3-replications); genetic line: SM007(3-replications); genetic line: SM012(3-replications); genetic line: SM013(3-replications); genetic line: SM022(3-replications); genetic line: SM024(3-replications); genetic line: SM027(3-replications); genetic line: SM041(3-replications); genetic line: SM043(3-replications); genetic line: SM044(3-replications); genetic line: SM046(3-replications); genetic line: SM061(3-replications); genetic line: SM063(3-replications); genetic line: SM073(3-replications); genetic line: SM074(3-replications); genetic line: SM079(3-replications); genetic line: SM085(3-replications); genetic line: SM088(3-replications); genetic line: SM089(3-replications); genetic line: SM116(3-replications); genetic line: SM130(3-replications); genetic line: SM135(3-replications); genetic line: SM136(3-replications); genetic line: SM140(3-replications); genetic line: SM141(3-replications); genetic line: SM146(3-replications); genetic line: SM152(3-replications); genetic line: SM155(3-replications); genetic line: SM160(3-replications); genetic line: SM167(3-replications); genetic line: SM169(3-replications); genetic line: SM173(3-replications); genetic line: SM177(3-replications); genetic line: SM184(3-replications); genetic line: SM200(3-replications); genetic line: Steptoe(3-replications); genetic line: Morex(3-replications)

2011-11-22 | E-GEOD-33394 | biostudies-arrayexpress

Transcriptome analysis of trichothecene-induced gene expression in barley

Project description:Fusarium Head Blight susceptible barley variety, Morex, was infected with deoxynivalenol production deficient mutant strain (GZT40) and wild type stains (Z3639) of Fusarium graminearum. The RNA was sampled at 48 and 96 hours after inoculation. and was used hybridize to Barley_1 GeneChip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jayanand Boddu. The equivalent experiment is BB52 at PLEXdb.] time: 48 hai. - Treated or untreated: WATER(3-replications); time: 48 hai. - Treated or untreated: MUTANT(3-replications); time: 48 hai. - Treated or untreated: WILD TYPE(3-replications); time: 96 hai. - Treated or untreated: WATER(3-replications); time: 96 hai. - Treated or untreated: MUTANT(3-replications); time: 96 hai. - Treated or untreated: WILD TYPE(3-replications)

2011-11-22 | E-GEOD-33398 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data