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Necrotic mutants in barley cv. Steptoe

ABSTRACT: FN044, FN211, FN242 and FN303 are the fast neutron generated mutants in cv. Steptoe background. These 4 mutants have lesion mimic phenotype and increase disease resistance to stem rust. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, ling zhang. The equivalent experiment is BB54 at PLEXdb.] line: nad1-1(3-replications); line: nad1-2(3-replications); line: nad1-3(3-replications); line: nad1-4 (FN242)(3-replications); line: Steptoe(3-replications)

ORGANISM(S): Hordeum vulgare

SUBMITTER: ling zhang

PROVIDER: E-GEOD-33399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A cation/proton-exchanging protein is a candidate for the barley NecS1 gene controlling necrosis and enhanced defense response to stem rust.

Zhang Ling L Lavery Lisa L Gill Upinder U Gill Kulvinder K Steffenson Brian B Yan Guiping G Chen Xianming X Kleinhofs Andris A

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 20081028 2

We characterized three lesion mimic necS1 (necrotic Steptoe) mutants, induced by fast neutron (FN) treatment of barley cultivar Steptoe. The three mutants are recessive and allelic. When infected with Puccinia graminis f. sp. tritici pathotypes MCC and QCC and P. graminis f. sp. secalis isolate 92-MN-90, all three mutants exhibited enhanced resistance compared to parent cultivar Steptoe. These results suggested that the lesion mimic mutants carry broad-spectrum resistance to stem rust. In order ...[more]

PMID: 18956175

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Genotype-dependent gene expression in barley

Project description:Comparison of mRNA accumulation in the seedling leaves of 8 unstressed barley cultivars ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB20 at PLEXdb.] genotype: Morex(3-replications); genotype: Steptoe(3-replications); genotype: OWB REC(3-replications); genotype: OWB DOM(3-replications); genotype: Barke(3-replications); genotype: Haruna Nijo(3-replications); genotype: Golden Promise(3-replications); genotype: Optic(3-replications)

2011-11-22 | E-GEOD-33393 | biostudies-arrayexpress

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Project description:Comparison of mRNA accumulation in segregating doubled haploid barley lines ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, . The equivalent experiment is BB21 at PLEXdb.] genetic line: SM004(3-replications); genetic line: SM007(3-replications); genetic line: SM012(3-replications); genetic line: SM013(3-replications); genetic line: SM022(3-replications); genetic line: SM024(3-replications); genetic line: SM027(3-replications); genetic line: SM041(3-replications); genetic line: SM043(3-replications); genetic line: SM044(3-replications); genetic line: SM046(3-replications); genetic line: SM061(3-replications); genetic line: SM063(3-replications); genetic line: SM073(3-replications); genetic line: SM074(3-replications); genetic line: SM079(3-replications); genetic line: SM085(3-replications); genetic line: SM088(3-replications); genetic line: SM089(3-replications); genetic line: SM116(3-replications); genetic line: SM130(3-replications); genetic line: SM135(3-replications); genetic line: SM136(3-replications); genetic line: SM140(3-replications); genetic line: SM141(3-replications); genetic line: SM146(3-replications); genetic line: SM152(3-replications); genetic line: SM155(3-replications); genetic line: SM160(3-replications); genetic line: SM167(3-replications); genetic line: SM169(3-replications); genetic line: SM173(3-replications); genetic line: SM177(3-replications); genetic line: SM184(3-replications); genetic line: SM200(3-replications); genetic line: Steptoe(3-replications); genetic line: Morex(3-replications)

2011-11-22 | E-GEOD-33394 | biostudies-arrayexpress

Necrotic mutants in barley cv. Steptoe

Project description:FN044, FN211, FN242 and FN303 are the fast neutron generated mutants in cv. Steptoe background. These 4 mutants have lesion mimic phenotype and increase disease resistance to stem rust. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, ling zhang. The equivalent experiment is BB54 at PLEXdb.]

2011-11-22 | GSE33399 | GEO

Mapping translocation breakpoints using a wheat microarray

Project description:Five RNA samples were used. The RNA source material was from wheat cytogenetic stocks from Dr. Adam Lukaszewski, University of California, Riverside. The lines used for this study are Pavon, Pavon Dt1BL, 1RS.1BL, 1B+5 and T-1 wheat samples. whole seedling minus starchy endosperm. Equal portions of RNA from unstressed and 48 hr drought-stressed seedling were combined to make one RNA sample. The person who produced the RNA was Prasanna R. Bhat in the TJ Close lab at University of California, Riverside. Everything downstream of RNA production was done at the UC Irvine DNA and Protein Microarray Facility (Denis Heck, manager) by Sriti Misra. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Prasanna Bhat. The equivalent experiment is TA10 at PLEXdb.] genetic line: Pavon(2-replications); genetic line: Pavon Dt1BL(2-replications); genetic line: 1RS.1BL(2-replications); genetic line: T-1(2-replications); genetic line: 1B+5(2-replications)

2011-08-30 | E-GEOD-31754 | biostudies-arrayexpress

Microarray of barley genes in wheat-barley chromosome addition lines

Project description:Transcript levels of barley genes were examined in the wheat-barley chromosome addition lines having one of six barley chromomes, 2H, 3H, 4H, 5H, 6H and 7H. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is BB8 at PLEXdb.] genotype: chr2H(3-replications); genotype: chr2HL(3-replications); genotype: chr3H(3-replications); genotype: chr4H(3-replications); genotype: chr5H(3-replications); genotype: chr6H(3-replications); genotype: chr6HL(3-replications); genotype: chr6HS(3-replications); genotype: chr7H(3-replications); genotype: Betzes(3-replications); genotype: Chinese Spring(3-replications)

2011-11-22 | E-GEOD-33415 | biostudies-arrayexpress

Drought stress in Wheat at grain filling stage

Project description:To provide a global study of transcriptome changes under drought stress, the gene expression levels of a durum wheat genotype (Triticum durum Desf. cultivar Creso) and two bread wheat genotypes (Triticum aestivum L. cultivar Chinese Spring -CS- and its deletion line CS_5AL-10) were investigated. The 5A chromosome deletion line (5AL-10) lacks the distal part (43%) of the long arm of chromosome 5A. Each genotype was subjected to two different levels of water stress at the grain filling stage. After anthesis, three different levels of soil water content (SWC) were induced as described below: control (CTRL; SWC=28%), moderate stress (MS; SWC=18%), and severe stress (SS; SWC=12.5%). For each sample, three biological replicates were performed, for a total of 27 hybridizations. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Alessio Aprile. The equivalent experiment is TA23 at PLEXdb.] genotype: Creso - stress condition: Control(3-replications); genotype: Creso - stress condition: Mild stress(3-replications); genotype: Creso - stress condition: Severe stress(3-replications); genotype: CS - stress condition: Control(3-replications); genotype: CS - stress condition: Mild stress(3-replications); genotype: CS - stress condition: Severe stress(3-replications); genotype: CS-5AL - stress condition: Control(3-replications); genotype: CS-5AL - stress condition: Mild stress(3-replications); genotype: CS-5AL - stress condition: Severe stress(3-replications)

2011-08-30 | E-GEOD-31759 | biostudies-arrayexpress

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Project description:Gene expression changes in a winter cultivar of barley in response to short and long term cold treatments ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Trevaskis. The equivalent experiment is BB94 at PLEXdb.] treatment: Non-vernalized (3-replications); treatment: Short-term cold (3-replications); treatment: Vernalized (3-replications); treatment: 1 day post-vernalized (3-replications)

2011-03-08 | E-GEOD-27821 | biostudies-arrayexpress

Long term cold responses in a winter barley

Project description:Gene expression changes maintained after vernalization ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Trevaskis. The equivalent experiment is BB95 at PLEXdb.] treatment: Non-vernalized(3-replications); treatment: Post-vernalized(3-replications)

2011-03-08 | E-GEOD-27822 | biostudies-arrayexpress

Functional Genomic Analysis Of Barley(Hordeum vulgare L.) Grain Protein Accumulation

Project description:Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Aravind Jukanti. The equivalent experiment is BB53 at PLEXdb.] developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 14 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: Karl - tissue type: Kernels(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Leaves(3-replications); developmental stage: 21 days past anthesis - genotype: 10-11 (NIL with high grain protein locus) - tissue type: Kernels(3-replications)

2011-11-22 | E-GEOD-33413 | biostudies-arrayexpress

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2010-05-12 | E-GEOD-21804 | biostudies-arrayexpress

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