Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comprehensive molecular characterization of the soybean rust disease [Embrapa-Soja - Iowa State Univ; 2005]

ABSTRACT: Experimental design: 2 genotypes: PI230970 (resistant USDA Plant Introduction (PI) line containing SBR Rpp2 resistance gene) & Embrapa-48 (susceptible Brazilian cultivar) 2 treatments: Soybean rust challenge & mock infection 3 replications 10 time points: 6, 12, 18, 24, 36, 48, 72, 96, 120, 168hai TOTAL: 120 Affymetrix GeneChip(R) Soybean Genome Arrays ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel. The equivalent experiment is GM2 at PLEXdb.] genotype: PI230970 - time: 006 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 006 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: Mock(3-replications)

ORGANISM(S): Glycine max

SUBMITTER: Martijn van de Mortel

PROVIDER: E-GEOD-33410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Distinct biphasic mRNA changes in response to Asian soybean rust infection.

van de Mortel Martijn M Recknor Justin C JC Graham Michelle A MA Nettleton Dan D Dittman Jaime D JD Nelson Rex T RT Godoy Cláudia V CV Abdelnoor Ricardo V RV Almeida Alvaro M R AM Baum Thomas J TJ Whitham Steven A SA

Molecular plant-microbe interactions : MPMI 20070801 8

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR-soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance ...[more]

PMID: 17722693

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Project description:A split-split-plot design with 144 experimental units (3 replications x 4 genotypes x 6 time points x 2 treatment types) was used to profile barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6). Barley leaves were harvested from inoculated and non-inoculated plants at 6 time points (0,8,16,20,24 and 32 hrs) after Bgh inoculation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB10 at PLEXdb.] genotype: Mla1 - time: 0 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 0 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla1 - time: 8 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 8 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla1 - time: 16 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 16 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla1 - time: 20 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 20 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla1 - time: 24 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 24 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla1 - time: 32 - Treated or untreated: Inoculated (3-replications); genotype: Mla1 - time: 32 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 0 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 0 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 8 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 8 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 16 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 16 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 20 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 20 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 24 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 24 - Treated or untreated: Non-inoculated(3-replications); genotype: m508 - time: 32 - Treated or untreated: Inoculated (3-replications); genotype: m508 - time: 32 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 0 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 0 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 8 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 8 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 16 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 16 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 20 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 20 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 24 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 24 - Treated or untreated: Non-inoculated(3-replications); genotype: Mla6 - time: 32 - Treated or untreated: Inoculated (3-replications); genotype: Mla6 - time: 32 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 0 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 0 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 8 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 8 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 16 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 16 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 20 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 20 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 24 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 24 - Treated or untreated: Non-inoculated(3-replications); genotype: 9472 - time: 32 - Treated or untreated: Inoculated (3-replications); genotype: 9472 - time: 32 - Treated or untreated: Non-inoculated(3-replications)

Project description:A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB4 at PLEXdb.] genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 0 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 8 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 16 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 20 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 24 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh 5874 - time: 32 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 0 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 8 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 16 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 20 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 24 hai(3-replications); genotype: CI 16137 (Mla1) - pathogen isolates: Bgh K1 - time: 32 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 0 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 8 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 16 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 20 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 24 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh 5874 - time: 32 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 0 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 8 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 16 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 20 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 24 hai(3-replications); genotype: CI 16151 (Mla6) - pathogen isolates: Bgh K1 - time: 32 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 0 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 8 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 16 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 20 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 24 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh 5874 - time: 32 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 0 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 8 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 16 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 20 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 24 hai(3-replications); genotype: CI 16155 (Mla13) - pathogen isolates: Bgh K1 - time: 32 hai(3-replications)

Dataset Information

Comprehensive molecular characterization of the soybean rust disease [Embrapa-Soja - Iowa State Univ; 2005]

Publications

Distinct biphasic mRNA changes in response to Asian soybean rust infection.

Similar Datasets

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