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Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types

ABSTRACT: Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a widespread and perhaps general feature of the gene expression program in human cells. 3 samples of non-malignant primary human leukocytes, one replicate each

ORGANISM(S): Homo sapiens

SUBMITTER: Julia Salzman

PROVIDER: E-GEOD-33772 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types.

Salzman Julia J Gawad Charles C Wang Peter Lincoln PL Lacayo Norman N Brown Patrick O PO

PloS one 20120201 2

Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-can ...[more]

PMID: 22319583

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