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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome of normal,paracancerous and cancerous colon tissues based on RNA-seq method

ABSTRACT: To support our research of colon cancer in human genome, we conducted massively parallel pyrosequencing of mRNAs (RNA-seq) using normal,paracancerouse and cancerous human colon tissues. We obtained a total of 29.9M reads from normal,33.0M reads from paracancerous and 36.5M reads from cancerous.The RNA-seq data derived from the sample illustrated the differencially expreesion genes among normal,paracancerous and cancerous colon tissues of human. 3 samples examined: normal tissue, paracancerous tissue, cancerous tissue.

ORGANISM(S): Homo sapiens

SUBMITTER: qianru ai

PROVIDER: E-GEOD-33782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome profiling of the cancer, adjacent non-tumor and distant normal tissues from a colorectal cancer patient by deep sequencing.

Wu Yan'an Y Wang Xuetao X Wu Fangbo F Huang Ruolei R Xue Fangqin F Liang Guantao G Tao Min M Cai Pengwei P Huang Yi Y

PloS one 20120808 8

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers in the world. A genome-wide screening of transcriptome dysregulation between cancer and normal tissue would provide insight into the molecular basis of CRC initiation and progression. Compared with microarray technology, which is commonly used to identify transcriptional changes, the recently developed RNA-seq technique has the ability to detect other abnormal regulations in the cancer transcriptome, such as alternative splici ...[more]

PMID: 22905095

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