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Effect of unilateral naris occlusion on gene profile in mouse olfactory mucosa.

ABSTRACT: Unilateral naris occlusion was performed the day after birth in three FVB strain mice. At 25 days of age olfactory mucosa was collected from open nasal fossa, the occluded nasal fossa and from three untreated mice. Total RNA was extracted and gene profiles among these three treatment conditions were compared. The goal of the study was to determine the effect of stimulus deprivation on the genetic profile of olfactory mucosa. There were three treatment conditions: open mucosa, occluded mucosa, and untreated mucosa. Each of these conditions had three biological replicates. Open and occluded however were repeated measures i.e. from the same individuals.

ORGANISM(S): Mus musculus

SUBMITTER: David Coppola

PROVIDER: E-GEOD-33833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The effects of unilateral naris occlusion on gene expression profiles in mouse olfactory mucosa.

Coppola David M DM Waggener Christopher T CT

Journal of molecular neuroscience : MN 20111221 3

Unilateral naris occlusion has been the method of choice for effecting stimulus deprivation in studies of olfactory plasticity. Early experiments emphasized the deleterious effects of this technique on the developing olfactory system while more recent studies have pointed to several apparently "compensatory" responses. However, the evidence for deprivation-induced compensatory processes in olfaction remains fragmentary. High-throughput methods such as microarray analysis can help fill the defici ...[more]

PMID: 22187364

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Effect of unilateral naris occlusion on gene profile in mouse olfactory mucosa.

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