Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Role of PKD2 in TCR-induced transcriptional reprogramming of naïve T cells


ABSTRACT: Comparison of transcriptional profile of TCR stimulated P14-TCR wild-type and P14-PKD2 null murine lymph node cells Lymph node cells from 3 biological replicates (3 wild-type and 3 PKD2 null mice) were isolated and left unstimulated or stimulated for 4 hours with antigenic peptide (gp33-41) prior to RNA extraction and hybridization to Affymetrix microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Maria Navarro 

PROVIDER: E-GEOD-33942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Protein kinase D2 has a restricted but critical role in T-cell antigen receptor signalling in mature T-cells.

Navarro Maria N MN   Sinclair Linda V LV   Feijoo-Carnero Carmen C   Clarke Rosemary R   Matthews Sharon A SA   Cantrell Doreen A DA  

The Biochemical journal 20120301 3


PKD (protein kinase D) 2 is a serine/threonine kinase activated by diacylglycerol in response to engagement of antigen receptors in lymphocytes. To explore PKD2 regulation and function in TCR (T-cell antigen receptor) signal transduction we expressed TCR complexes with fixed affinity for self antigens in the T-cells of PKD2-null mice or mice deficient in PKD2 catalytic activity. We also developed a single cell assay to quantify PKD2 activation as T-cells respond to developmental stimuli or engag  ...[more]

Similar Datasets

2012-02-29 | GSE33942 | GEO
2011-03-13 | E-GEOD-27092 | biostudies-arrayexpress
2020-04-02 | GSE147907 | GEO
| PRJNA989620 | ENA
2017-12-19 | PXD005833 | Pride
2018-02-08 | PXD007217 | Pride
| PRJEB58013 | ENA
| PRJEB57714 | ENA
2021-08-05 | PXD024852 | Pride
2021-07-16 | PXD024108 | Pride