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Dataset Information

Angiogenesis gene expression upon Notch activation in human vascular smooth muscle cells

ABSTRACT: VSMCs were cultured on Jag1 or IgG-coated plates as described above. RNA was extracted using RNA isolation kit (Qiagen). Reverse-transcription PCR was done with RNA-to-cDNA kit (Applied Biosystems). cDNA was run on a TaqMan Low-Density Array, human angiogenesis panel (Applied Biosystems , Cat#4378725), amplified on a 7900 HT Fast Real Time PCR system (Applied Biosystems) according to manufacturer’s instructions. Angiogenesis gene expression was screened for human aortic vascular smooth muscle cells that were plated for 18 hours on either Notch ligands (Jag1 or Jag2) as compared to appropriate IgG controls (IgG1 or IgG2).

ORGANISM(S): Homo sapiens

SUBMITTER: Sara Weis

PROVIDER: E-GEOD-34024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane.

Scheppke Lea L Murphy Eric A EA Zarpellon Alessandro A Hofmann Jennifer J JJ Merkulova Alona A Shields David J DJ Weis Sara M SM Byzova Tatiana V TV Ruggeri Zaverio M ZM Iruela-Arispe M Luisa ML Cheresh David A DA

Blood 20111201 9

Vascular development and angiogenesis initially depend on endothelial tip cell invasion, which is followed by a series of maturation steps, including lumen formation and recruitment of perivascular cells. Notch ligands expressed on the endothelium and their cognate receptors expressed on perivascular cells are involved in blood vessel maturation, though little is known regarding the Notch-dependent effectors that facilitate perivascular coverage of nascent vessels. Here, we report that vascular ...[more]

PMID: 22134168

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Dataset Information

Angiogenesis gene expression upon Notch activation in human vascular smooth muscle cells

Publications

Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane.

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