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Toh family 1

ABSTRACT: Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified. AB strain of zebrafish were used.The family consist of the parents and 2 male and female offspring each (total 6 samples). Dad's gDNA was used as reference sample for each family.

ORGANISM(S): Danio rerio

SUBMITTER: Woei Chang Liew

PROVIDER: E-GEOD-34336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polygenic sex determination system in zebrafish.

Liew Woei Chang WC Bartfai Richard R Lim Zijie Z Sreenivasan Rajini R Siegfried Kellee R KR Orban Laszlo L

PloS one 20120410 4

<h4>Background</h4>Despite the popularity of zebrafish as a research model, its sex determination (SD) mechanism is still unknown. Most cytogenetic studies failed to find dimorphic sex chromosomes and no primary sex determining switch has been identified even though the assembly of zebrafish genome sequence is near to completion and a high resolution genetic map is available. Recent publications suggest that environmental factors within the natural range have minimal impact on sex ratios of zebr ...[more]

PMID: 22506019

Similar Datasets

AB family 1

Project description:Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified. AB strain of zebrafish were used.The family consist of the parents and 2 male and female offspring each (total 6 samples). Dad's gDNA was used as reference sample for each family.

2012-04-16 | E-GEOD-34334 | biostudies-arrayexpress

AB family 2

2012-04-16 | E-GEOD-34335 | biostudies-arrayexpress

Toh family 2

2012-04-16 | E-GEOD-34337 | biostudies-arrayexpress

Array comparative genomic hybridization to identify genomic differences between male and female zebrafish.

Project description:This SuperSeries is composed of the following subset Series: GSE34334: AB family 1 GSE34335: AB family 2 GSE34336: Toh family 1 GSE34337: Toh family 2 Refer to individual Series

2012-04-16 | E-GEOD-34338 | biostudies-arrayexpress

Toh family 2

Project description:Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified.

2012-04-17 | GSE34337 | GEO

Toh family 1

Project description:Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified.

2012-04-17 | GSE34336 | GEO

AB family 2

Project description:Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified.

2012-04-17 | GSE34335 | GEO

AB family 1

Project description:Array comparative genomic hybridization was used to identify genomic differences between male and female zebrafish. No universal sex-linked CNVR was identified.

2012-04-17 | GSE34334 | GEO

Gender specific comparisons of gene expression during Zebrafish pectoral fin regeneration

Project description:To identify gender specific differences in gene expression during fin rgeneration, pectoral fins were amputated from both male and female adult fish. Fins were allowed to recover for 4 days in standard tank condtions then tissue was collected for RNA isolation and microarray analysis Pooled samples from male and females, injured and uninjured in biological trilicate.

2011-09-02 | E-GEOD-31871 | biostudies-arrayexpress

Visual automated fluorescence electrophoresis provides simultaneous quality, quantity, and molecular weight spectra for genomic DNA from archived neonatal blood spots

Project description:The Guthrie 903 card archived dried blood spots (DBS) are a unique but terminal resource amenable for individual and population wide genomic profiling. The limited amounts of DBS-derived genomic DNA (gDNA) can be whole-genome amplified (WGA) producing sufficient gDNA for genomic applications, albeit with variable success, and optimizing the isolation of high-quality DNA from these finite, low-yield specimens is essential. Visual automated fluorescence electrophoresis (VAFE) is a novel QC technology affording precise quality, quantity and molecular weight of double-stranded DNA from a single microliter of sample. The VAFE QC data were correlated with subsequent sample performance in PCR, sequencing, and high-density comparative genome hybridization array. The Swedish Repository of DBS samples collected on Guthrie 903 cards for neonatal screening of inborn diseases provided deidentified 3mm blood spot punches. There were total of eight (8) samples representing two (2) decades; 1970s: 1975, 1976, 1977, 1978; 1980s: 1980, 1982, 1984, 1986. gDNA was extracted and whole genome amplified prior to aCGH experiments using control female reference genomic DNA. The objective was to show that large rearrangements (e.g. loss of chrX in male samples) can be detected in WGA gDNA from blood spots >30 years old.

2013-07-01 | E-GEOD-44249 | biostudies-arrayexpress

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