Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Hh signaling-deficient OP9 stromal cells


ABSTRACT: In the bone marrow B cell development occurs in initimate and essential association with stromal cells. Known effects of stromal cells on B cell development have been shown to be mediated through direct contact and by soluble factors produced by stromal cells. Our findings suggest that cell-nonautonomous Hh signaling may play an important role in B cell lymphopoiesis. We therefore interrogated OP9 stromal cells for Hh-dependent transcripts that may confer B lymphopoietic identity. We generated OP9 stromal cells deficient in Hh signaling through targeting the non-redundant, pathway-obligate GPCR Smoothened. OP9 cells were transduced with RNAi pLKO.1-Puro lentiviral particles expressing a scrambled control shRNA (NT) or shRNA targeting Smoothened (Smo-kd). RNA from stable NT and Smo-kd OP9 cells were isolated in triplicate and global changes in transcripts were analyzed using the Affymetrix Mouse Exon 1.0 ST gene chip.

ORGANISM(S): Mus musculus

SUBMITTER: Stephen Desiderio 

PROVIDER: E-GEOD-34421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Non-cell-autonomous hedgehog signaling promotes murine B lymphopoiesis from hematopoietic progenitors.

Cooper Christopher L CL   Hardy Richard R RR   Reth Michael M   Desiderio Stephen S  

Blood 20120418 23


The role of hedgehog (Hh) signaling in B lymphopoiesis has remained unclear. We observed that the proliferation of pro-B cells in stromal cocultures was impaired by interruption of Hh signaling, prompting us to investigate whether the target of Hh antagonism was intrinsic or extrinsic to the B-lymphoid compartment. In the present study, using conditional deletion of the pathway activator gene Smo, we found that cell-autonomous Hh signaling is dispensable for B-cell development, B-lymphoid repopu  ...[more]

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