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Airway Epithelial miRNA Expression in Asthma

ABSTRACT: We performed genome-wide profiling of miRNA expression in the airway epithelial compartment in asthma to identify miRNA pathways associated with epithelial abnormalities using miRNA microarrays and real-time PCR. We also sought to identify the effect of inhaled corticosteroids (ICS) on airway epithelial miRNA expression Samples were obtained from airway epithelial cells by bronchoscopic brushing from three groups of subjects: Healthy Controls ( N=12), Steroid NaÃ¯ve Asthma (N=16), Steroid-requiring Asthma (N=19).

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Barczak

PROVIDER: E-GEOD-34466 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Airway epithelial miRNA expression is altered in asthma.

Solberg Owen D OD Ostrin Edwin J EJ Love Michael I MI Peng Jeffrey C JC Bhakta Nirav R NR Hou Lydia L Nguyen Christine C Solon Margaret M Nguyen Cindy C Barczak Andrea J AJ Zlock Lorna T LT Blagev Denitza P DP Finkbeiner Walter E WE Ansel K Mark KM Arron Joseph R JR Erle David J DJ Woodruff Prescott G PG

American journal of respiratory and critical care medicine 20120906 10

<h4>Rationale</h4>Changes in airway epithelial cell differentiation, driven in part by IL-13, are important in asthma. Micro-RNAs (miRNAs) regulate cell differentiation in many systems and could contribute to epithelial abnormalities in asthma.<h4>Objectives</h4>To determine whether airway epithelial miRNA expression is altered in asthma and identify IL-13-regulated miRNAs.<h4>Methods</h4>We used miRNA microarrays to analyze bronchial epithelial brushings from 16 steroid-naive subjects with asth ...[more]

PMID: 22955319

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