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Systematic RNA-seq analysis of the early events of CD4+ T cell activation

ABSTRACT: RNA-seq was used to look at the transcriptome changes and the early events of T cell receptor stimulation in CD4+ T cells CD4+ T cells were stimulated with immobilised anti-CD3/CD28 antibodies for 4 hours and RNA was extracted and subjected to RNA-seq analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Andrew Hutchins

PROVIDER: E-GEOD-34550 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Discovery and characterization of new transcripts from RNA-seq data in mouse CD4(+) T cells.

Hutchins Andrew Paul AP Poulain Stéphane S Fujii Hodaka H Miranda-Saavedra Diego D

Genomics 20120804 5

Despite the routine application of RNA-seq technology to profile cellular transcriptomes and report novel splice variants, the identification and validation of new transcripts remain underexplored. We prepared two RNA-seq libraries from resting and T cell receptor-stimulated mouse CD4(+) T cells. Transcripts unknown to Ensembl represent as much as 5% of the assembled transcripts and are robustly expressed but do not show the same degree of evolutionary conservation or exon distribution of known ...[more]

PMID: 22884873

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