Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptome of 3 developmental stages of Colletotrichum graminicola during infection of Zea mays leaf sheaths

ABSTRACT: Transcriptome of 3 developmental stages of Colletotrichum graminicola during infection of Zea mays leaf sheaths 3 biological replicates per stage. The three stages are: pre-penetration appressoria (PA), early biotrophic phase (BP), and the switch from biotrophy to necrotrophy (NP). Each biological replicate of the first stage, the pre-penetration appressoria, was sequenced to a 2-fold greater depth due to its lower representation in the samples.

ORGANISM(S): Glomerella graminicola

SUBMITTER: Lisa Vaillancourt

PROVIDER: E-GEOD-34632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses.

O'Connell Richard J RJ Thon Michael R MR Hacquard Stéphane S Amyotte Stefan G SG Kleemann Jochen J Torres Maria F MF Damm Ulrike U Buiate Ester A EA Epstein Lynn L Alkan Noam N Altmüller Janine J Alvarado-Balderrama Lucia L Bauser Christopher A CA Becker Christian C Birren Bruce W BW Chen Zehua Z Choi Jaeyoung J Crouch Jo Anne JA Duvick Jonathan P JP Farman Mark A MA Gan Pamela P Heiman David D Henrissat Bernard B Howard Richard J RJ Kabbage Mehdi M Koch Christian C Kracher Barbara B Kubo Yasuyuki Y Law Audrey D AD Lebrun Marc-Henri MH Lee Yong-Hwan YH Miyara Itay I Moore Neil N Neumann Ulla U Nordström Karl K Panaccione Daniel G DG Panstruga Ralph R Place Michael M Proctor Robert H RH Prusky Dov D Rech Gabriel G Reinhardt Richard R Rollins Jeffrey A JA Rounsley Steve S Schardl Christopher L CL Schwartz David C DC Shenoy Narmada N Shirasu Ken K Sikhakolli Usha R UR Stüber Kurt K Sukno Serenella A SA Sweigard James A JA Takano Yoshitaka Y Takahara Hiroyuki H Trail Frances F van der Does H Charlotte HC Voll Lars M LM Will Isa I Young Sarah S Zeng Qiandong Q Zhang Jingze J Zhou Shiguo S Dickman Martin B MB Schulze-Lefert Paul P Ver Loren van Themaat Emiel E Ma Li-Jun LJ Vaillancourt Lisa J LJ

Nature genetics 20120812 9

Colletotrichum species are fungal pathogens that devastate crop plants worldwide. Host infection involves the differentiation of specialized cell types that are associated with penetration, growth inside living host cells (biotrophy) and tissue destruction (necrotrophy). We report here genome and transcriptome analyses of Colletotrichum higginsianum infecting Arabidopsis thaliana and Colletotrichum graminicola infecting maize. Comparative genomics showed that both fungi have large sets of pathog ...[more]

PMID: 22885923

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Project description:Colletotrichum graminicola is a hemibiotrophic fungal pathogen that causes maize anthracnose disease. It progresses through three recognizable phases of pathogenic development *in planta*: melanized appressoria that form on the host surface prior to penetration; biotrophy, characterized by colonization of living host cells; and necrotrophy, characterized by host cell death and symptom development. An improved filtering algorithm and a Mixed Effects Generalized Linear Model (GLM) were developed and applied to an existing Illumina transcriptome dataset, substantially increasing the statistical power of the analysis of *C. graminicola* gene expression during infection and colonization. Additionally, the *in planta* transcriptome of the wild-type was compared with that of a mutant strain impaired in the establishment of biotrophy, allowing detailed dissection of events occurring specifically during penetration, and during early versus late biotrophy. Results indicated that there is a continuum of activities that occur during colonization of maize by *C. graminicola*, and that boundaries drawn between the three recognizable phases are artificial. More than 2000 fungal genes were differentially transcribed in waves during appressorial maturation, penetration, and colonization. Secreted proteins and membrane receptors were over-represented among the differentially expressed genes, suggesting that the fungus engages in an intimate and dynamic conversation with the host, beginning prior to penetration. This communication process is likely to involve reception of plant signals that trigger subsequent developmental progress in the fungus, as well as the production of signals that induce responses in the host. Later phases of biotrophy were more similar to necrotrophy, with increased production of secreted proteases, inducers of plant cell death, hydrolases, and membrane bound transporters for the uptake and egress of potential toxins, signals, and nutrients. The differentially expressed genes could be used as landmarks to more accurately identify developmental progress in compatible versus incompatible interactions involving genetic variants of both host and pathogen.

Dataset Information

Transcriptome of 3 developmental stages of Colletotrichum graminicola during infection of Zea mays leaf sheaths

Publications

Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses.

Similar Datasets

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