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Circulating microRNAs to predict neurological outcome after sudden cardiac arrest

ABSTRACT: Studies of miRNA profiling to predict outcome in cardiac arrest patients treated with therapeutic hypothermia (TH) Venous blood was collected in citrated tubes prior discharge. Plasma was harvested by centrifugation and stored at -80Â°C until assayed. Identical volumes of plasma from the 14 patients of a group (cerebral performance category (CPC): 1-2 or CPC: 3-5) were pooled to reach a final volume of 400ÂµL for each group. The two pools were processed conjointly. Total RNA was extracted using miRVana isolation kit (Applied Biosystems), dephosphorylated and labeled using miRNA Complete Labeling and hybridization kit (Agilent). Hybridization was performed on miRNA Human Microarray Release 12.0 slides (Agilent). 4 arrays per pool were hybridized. Scanning was achieved with the Genepix 4000B Scanner (Molecular Devices, Sunnyvale, USA). Raw data were acquired with the Genepix Pro software (Molecular Devices).

ORGANISM(S): Homo sapiens

SUBMITTER: Yvan Devaux

PROVIDER: E-GEOD-34643 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Circulating microRNAs after cardiac arrest.

Stammet Pascal P Goretti Emeline E Vausort Mélanie M Zhang Lu L Wagner Daniel R DR Devaux Yvan Y

Critical care medicine 20121201 12

<h4>Objective</h4>Prediction of clinical outcome after cardiac arrest is clinically important. While the potential of circulating microRNAs as biomarkers of acute coronary syndromes is an active field of investigation, it is unknown whether microRNAs are associated with outcome in cardiac arrest patients.<h4>Design</h4>Prospective, single-center proof-of-concept study.<h4>Setting</h4>Eighteen-bed adult general intensive care unit of an academic tertiary care hospital in Luxembourg.<h4>Patients</ ...[more]

PMID: 22890253

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