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RNAi knock down of LEDGF transcript from the PSIP1 gene in human 293T cells reveals a role for LEDFG/p75 in targeting HIV integration

ABSTRACT: The LEDGF transcript from the PSIP1 gene was knocked down using RNAi technology. The resulting 293T derived cell line (293TsiLL) was compared to a control cell line (293TsiScram) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Experiment Overall Design: 2 cell lines were used, 293TsiLL (experimental) and 293TsiScram (control). 4 independent cultures of each cell line were used for independent RNA extractions, labeling reactions and array hybridizations.

ORGANISM(S): Homo sapiens

SUBMITTER: Chris Hoffmann

PROVIDER: E-GEOD-3485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A role for LEDGF/p75 in targeting HIV DNA integration.

Ciuffi Angela A Llano Manuel M Poeschla Eric E Hoffmann Christian C Leipzig Jeremy J Shinn Paul P Ecker Joseph R JR Bushman Frederic F

Nature medicine 20051127 12

HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus ...[more]

PMID: 16311605

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