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HNSCC Cells: ctrl vs six tumour lethal miRNAs transfected

ABSTRACT: Some microRNAs have antiproliferative effects in cells and are believed to act as tumour suppressors. We used a retroviral human microRNA expression library to identify six microRNAs, miR-181a, miR-323, miR-326, miR-342, miR345 and miR-371, that induce antiproliferative effects in head and neck squamous cell carcinoma (HNSCC), but do not in normal oral keratinocytes. By gene expression profilling we showed that the ataxia telangiectasia mutated (ATM) gene is a common target for three of these microRNAs. Transcriptional profilling of head and neck cancer cells comparing transiently transfected with either one of the six miRNAs to the cells transfected with a control vector. One-condition experiment, ctrl vs miRNA transfected, Biological replicates: 2 replicates for each miRNA transfected, 4 replicates for ctrl transfected

ORGANISM(S): Homo sapiens

SUBMITTER: Daoud Sie 

PROVIDER: E-GEOD-34881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of lethal microRNAs specific for head and neck cancer.

Lindenbergh-van der Plas Marlon M   Martens-de Kemp Sanne R SR   de Maaker Michiel M   van Wieringen Wessel N WN   Ylstra Bauke B   Agami Reuven R   Cerisoli Francesco F   Leemans C René CR   Braakhuis Boudewijn J M BJ   Brakenhoff Ruud H RH  

Clinical cancer research : an official journal of the American Association for Cancer Research 20130813 20

<h4>Purpose</h4>The prognosis of head and neck squamous cell carcinomas (HNSCC) remains disappointing and the development of novel anti-cancer agents is urgently awaited. We identified by a functional genetic screen microRNAs that are selectively lethal for head and neck cancer cells but not for normal cells. We further investigated the genes targeted by these microRNAs.<h4>Experimental design</h4>A retroviral expression library of human microRNAs was introduced in HNSCC cell lines and normal or  ...[more]

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