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Effects of methotrexate on S3 Drosophila cells and Drosophila ovaries

ABSTRACT: Comparison of transcript abundance between control (untreated) and methotrexate treated S3 Drosophila cells and ovaries disected from female flies. S3 cells were exposed to 0 or 5.2 X 10^-8 M MTX for 4 days then harvested. Flies were exposed to 0 or 5 ppm MTX for 5 days then ovaries were dissected. RNA was extracted from S3 cells and ovaries. 4 S3 microarrays with one dye-swap and 2 ovary microarrays swapping dyes were hybridized to spotted cDNA microarrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Virginia Walker

PROVIDER: E-GEOD-3495 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The effects of methotrexate on Drosophila development, female fecundity, and gene expression.

Affleck Joslynn G JG Neumann Katerina K Wong Lily L Walker Virginia K VK

Toxicological sciences : an official journal of the Society of Toxicology 20051109 2

Methotrexate (MTX), a synthetic folate analog, is a tight-binding inhibitor of dihydrofolate reductase (DHFR), a key enzyme for the biosynthesis of purines, thymidylate, and several amino acids. As a consequence, MTX decreases titres of reduced folates, interferes with DNA synthesis, and results in the arrest of rapidly proliferating cells, making it a drug of choice for the treatment of a variety of cancers and auto-immune disorders. MTX is also a known teratogen in all higher animals tested, b ...[more]

PMID: 16280378

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