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Expression profiling of primary wild type (WT), Ezh2-null and Eed-null murine MLL-AF9 AML

ABSTRACT: We evaluated gene expression changes in murine leukemia caused by retroviral overexpression of MLL-AF9. We compared wild-type (WT) leukemia cells with mutant leukemia cells after cre-mediated inactivation of homozygous conditional alleles for Ezh2 or Eed, both of which are components of the Polycomb Repressive Complex2. For WT cells, 3 biological replicates were hybridized. For Ezh2-null cells, 4 biological replicates were hybridized. For Eed-cells, 3 biological replicates were hybridized.

ORGANISM(S): Mus musculus

SUBMITTER: Amit Sinha

PROVIDER: E-GEOD-34959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polycomb repressive complex 2 is required for MLL-AF9 leukemia.

Neff Tobias T Sinha Amit U AU Kluk Michael J MJ Zhu Nan N Khattab Mohamed H MH Stein Lauren L Xie Huafeng H Orkin Stuart H SH Armstrong Scott A SA

Proceedings of the National Academy of Sciences of the United States of America 20120306 13

A growing body of data suggests the importance of epigenetic mechanisms in cancer. Polycomb repressive complex 2 (PRC2) has been implicated in self-renewal and cancer progression, and its components are overexpressed in many cancers. However, its role in cancer development and progression remains unclear. We used conditional alleles for the PRC2 components enhancer of zeste 2 (Ezh2) and embryonic ectoderm development (Eed) to characterize the role of PRC2 function in leukemia development and pro ...[more]

PMID: 22396593

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