Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of human Th17 cell differentiation

ABSTRACT: The study aims at identifying transcriptional changes induced by in vitro polarization of human cord blood CD4+ cells towards Th17 subtype with combination of IL6, IL1b and TGFb by using timeseries data. In this study, we identified gene expression changes characterizing early stages of human Th17 cell differentiation program through genome-wide gene expression profiling. Primary T helper cells isolated from umbilical cord blood were used to construct detailed kinetic patterns of gene expression after initiation of Th17 differentiation with IL1b, IL6 and TGFb. The dataset described provides the starting point for defining the gene regulatory networks and identifying new candidates regulating the Th17 differentiation in human. Altogether 57 samples were analyzed representing 3 biological replicates of timeseries data (0, 0.5, 1, 2, 4, 6, 12, 24, 48 and 72 hours) of Th17 polarized cells and control Th0 cells

ORGANISM(S): Homo sapiens

SUBMITTER: Soile Tuomela

PROVIDER: E-GEOD-35103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of early gene expression changes during human Th17 cell differentiation.

Tuomela Soile S Salo Verna V Tripathi Subhash K SK Chen Zhi Z Laurila Kirsti K Gupta Bhawna B Äijö Tarmo T Oikari Lotta L Stockinger Brigitta B Lähdesmäki Harri H Lahesmaa Riitta R

Blood 20120427 23

Th17 cells play an essential role in the pathogenesis of autoimmune and inflammatory diseases. Most of our current understanding on Th17 cell differentiation relies on studies carried out in mice, whereas the molecular mechanisms controlling human Th17 cell differentiation are less well defined. In this study, we identified gene expression changes characterizing early stages of human Th17 cell differentiation through genome-wide gene expression profiling. CD4(+) cells isolated from umbilical cor ...[more]

PMID: 22544700

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Dataset Information

Gene expression profiling of human Th17 cell differentiation

Publications

Identification of early gene expression changes during human Th17 cell differentiation.

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