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Development of gene expression signatures in methylotrophic grown Bacillus methanolicus

ABSTRACT: A total gene expression approach was applied to study the methylotrophic nature of B. methanolicus by comparing the gene expression in bacteria grown methylotropic compared to non-methylotrophic. Genes of interest with different gene expression were quantified in the same RNA samples by real-time PCR, confirming the results found in the microarray experiment. Genes of special interest that are expressed higher when grown methylotrophic, were the RuMP pathway genes located on the pBM19. Bacillus methanolicus was grown in minimal media with either methanol or mannitol as carbon source. The experiment was preformed in triplicate, with bacterial cultures grown on 3 different days.

ORGANISM(S): Bacillus methanolicus

SUBMITTER: Tonje Heggeset

PROVIDER: E-GEOD-35298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol.

Heggeset Tonje M B TM Krog Anne A Balzer Simone S Wentzel Alexander A Ellingsen Trond E TE Brautaset Trygve T

Applied and environmental microbiology 20120518 15

Bacillus methanolicus can utilize methanol as its sole carbon and energy source, and the scientific interest in this thermotolerant bacterium has focused largely on exploring its potential as a biocatalyst for the conversion of methanol into L-lysine and L-glutamate. We present here the genome sequences of the important B. methanolicus model strain MGA3 (ATCC 53907) and the alternative wild-type strain PB1 (NCIMB13113). The physiological diversity of these two strains was demonstrated by a compa ...[more]

PMID: 22610424

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Development of gene expression signatures in methylotrophic grown Bacillus methanolicus

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