Unknown,Transcriptomics,Genomics,Proteomics

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Expression from early pre-hematopoietic progenitors from mouse embryo


ABSTRACT: Hematopoietic Stem Cells (HSC) are originated during embryonic development from endothelial-like cells located in the ventral side of the dorsal aorta around day E10-12 of murine development. This region is called AGM for Aorta/Gonad/Mesonephros and refers to the tissues around the hemogenic aorta. Cells that emerge from the endothelium and show hematopoietic traits can be distinguished by the expression of the c-kit receptor and finally acquire the CD45 marker. AGM regions were obtained from E11.5 embryos by dissection and digested with 0.1% collagenase. Cells were stained with anti-CD31, anti-ckit, anti-CD45 and anti-Ter119 antibodies. Sorting of the CD31+CD45-Ter119- population was performed, and cells were separated into c-kit+ and c-kit-. 3 replicates each of c-kit+ and c-kit- cells.

ORGANISM(S): Mus musculus

SUBMITTER: Anna Bigas 

PROVIDER: E-GEOD-35395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Hematopoietic stem cell development requires transient Wnt/β-catenin activity.

Ruiz-Herguido Cristina C   Guiu Jordi J   D'Altri Teresa T   Inglés-Esteve Julia J   Dzierzak Elaine E   Espinosa Lluis L   Bigas Anna A  

The Journal of experimental medicine 20120716 8


Understanding how hematopoietic stem cells (HSCs) are generated and the signals that control this process is a crucial issue for regenerative medicine applications that require in vitro production of HSC. HSCs emerge during embryonic life from an endothelial-like cell population that resides in the aorta-gonad-mesonephros (AGM) region. We show here that β-catenin is nuclear and active in few endothelial nonhematopoietic cells closely associated with the emerging hematopoietic clusters of the emb  ...[more]

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